Physical map of the vitopine Ti plasmid pTiS4

Jean Claude Gérard, Jean Canaday, Ernö Szegedi, Henri de la Salle, Léon Otten

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Abstract

Within the Agrobacterium vitis group the vitopine strains represent a special subclass. Vitopine bacteria carry Ti plasmids with little or no homology with the well-characterized T-DNAs of Agrobacterium tumefaciens or Agrobacterium rhizogenes. The 262-kb Ti plasmid of the vitopine strain S4 was cloned and mapped. Homology studies with the octopine Ti plasmid pTiAch5, the nopaline Ti plasmid pTiC58, and the agropine/mannopine Ri plasmid pRiHRI identified several regions of homology. The origin of replication was localized to within 2.5 kb.

Original languageEnglish
Pages (from-to)146-156
Number of pages11
JournalPlasmid
Volume28
Issue number2
DOIs
Publication statusPublished - Sep 1992

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ASJC Scopus subject areas

  • Molecular Biology

Cite this

Gérard, J. C., Canaday, J., Szegedi, E., de la Salle, H., & Otten, L. (1992). Physical map of the vitopine Ti plasmid pTiS4. Plasmid, 28(2), 146-156. https://doi.org/10.1016/0147-619X(92)90045-C