Phylogeny of Mycoplasma bovis isolates from Hungary based on multi locus sequence typing and multiple-locus variable-number tandem repeat analysis

Kinga M. Sulyok, Zsuzsa Kreizinger, Lilla Fekete, S. Jánosi, Nóra Schweitzer, Ibolya Turcsányi, L. Makrai, K. Erdélyi, Miklós Gyuranecz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Mycoplasma bovis is an important pathogen causing pneumonia, mastitis and arthritis in cattle worldwide. As this agent is primarily transmitted by direct contact and spread through animal movements, efficient genotyping systems are essential for the monitoring of the disease and for epidemiological investigations. The aim of this study was to compare and evaluate the multi locus sequence typing (MLST) and the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) through the genetic characterization of M. bovis isolates from Hungary. Results: Thirty one Hungarian M. bovis isolates grouped into two clades by MLST. Two strains had the same sequence type (ST) as reference strain PG45, while the other twenty nine Hungarian isolates formed a novel clade comprising five subclades. Isolates originating from the same herds had the same STs except for one case. The same isolates formed two main clades and several subclades and branches by MLVA. One clade contained the reference strain PG45 and three isolates, while the other main clade comprised the rest of the strains. Within-herd strain divergence was also detected by MLVA. Little congruence was found between the results of the two typing systems. Conclusions: MLST is generally considered an intermediate scale typing method and it was found to be discriminatory among the Hungarian M. bovis isolates. MLVA proved to be an appropriate fine scale typing tool for M. bovis as this method was able to distinguish closely related strains isolated from the same farm. We recommend the combined use of the two methods for the genotyping of M. bovis isolates. Strains have to be characterized first by MLST followed by the fine scale typing of identical STs with MLVA.

Original languageEnglish
Article number108
JournalBMC Veterinary Research
Volume10
DOIs
Publication statusPublished - May 7 2014

Fingerprint

Mycoplasma bovis
Minisatellite Repeats
Hungary
Phylogeny
phylogeny
genotyping
Epidemiological Monitoring
herds
Mastitis
disease surveillance
arthritis
Arthritis
direct contact
multiple-locus variable number tandem-repeat analysis
multilocus sequence typing
Pneumonia
pneumonia
mastitis
methodology
farms

Keywords

  • Cattle
  • Genotyping
  • MLST
  • MLVA
  • Mycoplasma bovis
  • Phylogeny
  • VNTR

ASJC Scopus subject areas

  • veterinary(all)
  • Medicine(all)

Cite this

Phylogeny of Mycoplasma bovis isolates from Hungary based on multi locus sequence typing and multiple-locus variable-number tandem repeat analysis. / Sulyok, Kinga M.; Kreizinger, Zsuzsa; Fekete, Lilla; Jánosi, S.; Schweitzer, Nóra; Turcsányi, Ibolya; Makrai, L.; Erdélyi, K.; Gyuranecz, Miklós.

In: BMC Veterinary Research, Vol. 10, 108, 07.05.2014.

Research output: Contribution to journalArticle

Sulyok, Kinga M. ; Kreizinger, Zsuzsa ; Fekete, Lilla ; Jánosi, S. ; Schweitzer, Nóra ; Turcsányi, Ibolya ; Makrai, L. ; Erdélyi, K. ; Gyuranecz, Miklós. / Phylogeny of Mycoplasma bovis isolates from Hungary based on multi locus sequence typing and multiple-locus variable-number tandem repeat analysis. In: BMC Veterinary Research. 2014 ; Vol. 10.
@article{2ba66139cf84471a8b84aa2f948d97d2,
title = "Phylogeny of Mycoplasma bovis isolates from Hungary based on multi locus sequence typing and multiple-locus variable-number tandem repeat analysis",
abstract = "Background: Mycoplasma bovis is an important pathogen causing pneumonia, mastitis and arthritis in cattle worldwide. As this agent is primarily transmitted by direct contact and spread through animal movements, efficient genotyping systems are essential for the monitoring of the disease and for epidemiological investigations. The aim of this study was to compare and evaluate the multi locus sequence typing (MLST) and the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) through the genetic characterization of M. bovis isolates from Hungary. Results: Thirty one Hungarian M. bovis isolates grouped into two clades by MLST. Two strains had the same sequence type (ST) as reference strain PG45, while the other twenty nine Hungarian isolates formed a novel clade comprising five subclades. Isolates originating from the same herds had the same STs except for one case. The same isolates formed two main clades and several subclades and branches by MLVA. One clade contained the reference strain PG45 and three isolates, while the other main clade comprised the rest of the strains. Within-herd strain divergence was also detected by MLVA. Little congruence was found between the results of the two typing systems. Conclusions: MLST is generally considered an intermediate scale typing method and it was found to be discriminatory among the Hungarian M. bovis isolates. MLVA proved to be an appropriate fine scale typing tool for M. bovis as this method was able to distinguish closely related strains isolated from the same farm. We recommend the combined use of the two methods for the genotyping of M. bovis isolates. Strains have to be characterized first by MLST followed by the fine scale typing of identical STs with MLVA.",
keywords = "Cattle, Genotyping, MLST, MLVA, Mycoplasma bovis, Phylogeny, VNTR",
author = "Sulyok, {Kinga M.} and Zsuzsa Kreizinger and Lilla Fekete and S. J{\'a}nosi and N{\'o}ra Schweitzer and Ibolya Turcs{\'a}nyi and L. Makrai and K. Erd{\'e}lyi and Mikl{\'o}s Gyuranecz",
year = "2014",
month = "5",
day = "7",
doi = "10.1186/1746-6148-10-108",
language = "English",
volume = "10",
journal = "BMC Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Phylogeny of Mycoplasma bovis isolates from Hungary based on multi locus sequence typing and multiple-locus variable-number tandem repeat analysis

AU - Sulyok, Kinga M.

AU - Kreizinger, Zsuzsa

AU - Fekete, Lilla

AU - Jánosi, S.

AU - Schweitzer, Nóra

AU - Turcsányi, Ibolya

AU - Makrai, L.

AU - Erdélyi, K.

AU - Gyuranecz, Miklós

PY - 2014/5/7

Y1 - 2014/5/7

N2 - Background: Mycoplasma bovis is an important pathogen causing pneumonia, mastitis and arthritis in cattle worldwide. As this agent is primarily transmitted by direct contact and spread through animal movements, efficient genotyping systems are essential for the monitoring of the disease and for epidemiological investigations. The aim of this study was to compare and evaluate the multi locus sequence typing (MLST) and the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) through the genetic characterization of M. bovis isolates from Hungary. Results: Thirty one Hungarian M. bovis isolates grouped into two clades by MLST. Two strains had the same sequence type (ST) as reference strain PG45, while the other twenty nine Hungarian isolates formed a novel clade comprising five subclades. Isolates originating from the same herds had the same STs except for one case. The same isolates formed two main clades and several subclades and branches by MLVA. One clade contained the reference strain PG45 and three isolates, while the other main clade comprised the rest of the strains. Within-herd strain divergence was also detected by MLVA. Little congruence was found between the results of the two typing systems. Conclusions: MLST is generally considered an intermediate scale typing method and it was found to be discriminatory among the Hungarian M. bovis isolates. MLVA proved to be an appropriate fine scale typing tool for M. bovis as this method was able to distinguish closely related strains isolated from the same farm. We recommend the combined use of the two methods for the genotyping of M. bovis isolates. Strains have to be characterized first by MLST followed by the fine scale typing of identical STs with MLVA.

AB - Background: Mycoplasma bovis is an important pathogen causing pneumonia, mastitis and arthritis in cattle worldwide. As this agent is primarily transmitted by direct contact and spread through animal movements, efficient genotyping systems are essential for the monitoring of the disease and for epidemiological investigations. The aim of this study was to compare and evaluate the multi locus sequence typing (MLST) and the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) through the genetic characterization of M. bovis isolates from Hungary. Results: Thirty one Hungarian M. bovis isolates grouped into two clades by MLST. Two strains had the same sequence type (ST) as reference strain PG45, while the other twenty nine Hungarian isolates formed a novel clade comprising five subclades. Isolates originating from the same herds had the same STs except for one case. The same isolates formed two main clades and several subclades and branches by MLVA. One clade contained the reference strain PG45 and three isolates, while the other main clade comprised the rest of the strains. Within-herd strain divergence was also detected by MLVA. Little congruence was found between the results of the two typing systems. Conclusions: MLST is generally considered an intermediate scale typing method and it was found to be discriminatory among the Hungarian M. bovis isolates. MLVA proved to be an appropriate fine scale typing tool for M. bovis as this method was able to distinguish closely related strains isolated from the same farm. We recommend the combined use of the two methods for the genotyping of M. bovis isolates. Strains have to be characterized first by MLST followed by the fine scale typing of identical STs with MLVA.

KW - Cattle

KW - Genotyping

KW - MLST

KW - MLVA

KW - Mycoplasma bovis

KW - Phylogeny

KW - VNTR

UR - http://www.scopus.com/inward/record.url?scp=84900021217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900021217&partnerID=8YFLogxK

U2 - 10.1186/1746-6148-10-108

DO - 10.1186/1746-6148-10-108

M3 - Article

C2 - 24885530

AN - SCOPUS:84900021217

VL - 10

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

M1 - 108

ER -