Phylogenetic Diversity of Bacterial Communities Associated with Sulfurous Karstic Well Waters of a Hungarian Spa

Roland Miseta, Márton Palatinszky, Judit Makk, Károly Márialigeti, Andrea K. Borsodi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The composition of bacterial communities associated with waters of one thermal and three sulfurous lukewarm wells of cavernous limestone aquifers in the Southwestern part of Hungary was studied in 2007 and 2008 by microscopy and culture-independent clone library analysis targeting the 16S rRNA gene. The presence of Thiothrix-like filamentous bacteria was observed by microscopy in the lukewarm samples. From the clone libraries, a high proportion of phylotypes belonging to Proteobacteria (Rhodobacter, Sphingopyxis, Phenylobacterium, Ochrobactrum, Methylobacterium, Sulfuritalea, Thiobacillus, Limnobacter, Acidovorax, Xanthomonadaceae, Thiothrix, Lysobacter, Pseudoxanthomonas, Desulfopila, Desulfocapsa, Desulforhopalus, Sulfuricurvum, Sulfurimonas, Sulfurospirillum) was revealed. In addition, phylotypes of Acidobacteria, Chloroflexi, Sphingobacteria, Clostridia, Aquificae, Deferribacteres and Chlorobi were detected. On the basis of their habitat preference 21 out of a total of 39 different phylotypes represented mesophilic and thermophilic sulfur bacteria. From the lukewarm samples large numbers of clones were affiliated with uncultured Epsilonproteobacteria clones common in sulfurous hydrothermal vents. From the thermal sample, several clones were in the closest relationship to the Sulfuritalea hydrogenivorans, a novel sulfur-oxidizing Betaproteobacterium.

Original languageEnglish
Pages (from-to)101-113
Number of pages13
JournalGeomicrobiology Journal
Volume29
Issue number2
DOIs
Publication statusPublished - Mar 1 2012

Keywords

  • 16S rDNA sequence analysis
  • bacterial diversity
  • clone library
  • sulfurous well water

ASJC Scopus subject areas

  • Microbiology
  • Environmental Chemistry
  • Environmental Science(all)
  • Earth and Planetary Sciences (miscellaneous)

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