Phylogenetic and metabolic bacterial diversity of Phragmites australis periphyton communities in two Hungarian soda ponds

Anna Rusznyák, Péter Vladár, Gitta Szabó, Károly Márialigeti, Andrea K. Borsodi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bacterial diversity of reed (Phragmites australis) periphyton communities of Kelemen-szék and Nagy-Vadas (two Hungarian soda ponds) was investigated using molecular cloning and cultivation-based techniques. The majority of the 80 Kelemen-szék and 72 Nagy-Vadas bacterial isolates proved to be moderately halophilic and alkaliphilic. A great proportion of the isolates showed phosphatase and urease activity, utilized aesculin, citrate and certain biopolymers (e.g., gelatine and tween 80). Partial 16S rDNA sequence analysis of 33 Kelemen-szék and 20 Nagy-Vadas ARDRA group representatives showed Gram-positive (Nesterenkonia, Cellulomonas, Dietzia, Bacillus and Planococcus) dominance at both sampling sites. Species of the genera Acidovorax, Hydrogenophaga (β-Proteobacteria) and Flavobacterium, Sphingobacterium (Bacteroidetes) were represented only from Kelemen-szék. Altogether 16 isolates showed low sequence similarity with yet described bacteria and may represent novel taxa. Screening of the 16S rRNA gene libraries of 129 Kelemen-szék and 158 Nagy-Vadas clones resulted in 30 and 28 different ARDRA groups, respectively. Sequence analysis revealed a Gram-negative (Rheinheimera, Aquimonas, Cellvibrio, Flavobacterium and Sphingobacterium) dominated phylogenetic diversity. A high number of the clones were affiliated with uncultured bacterial clones described from diverse environmental samples.

Original languageEnglish
Pages (from-to)763-773
Number of pages11
JournalExtremophiles
Volume12
Issue number6
DOIs
Publication statusPublished - Nov 1 2008

Keywords

  • 16S rRNA sequence analysis
  • Biofilm
  • Cloning
  • Cultivation
  • Phragmites australis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Medicine

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