Phylogenetic and functional analysis of sequence variation of human papillomavirus type 31 E6 and E7 oncoproteins

Annamária Ferenczi, Eszter Gyöngyösi, Anita Szalmás, Brigitta László, József Kónya, G. Veress

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

High-risk human papillomaviruses (HPV) are the causative agents of cervical and other anogenital cancers as well as a subset of head and neck cancers. The E6 and E7 oncoproteins of HPV contribute to oncogenesis by associating with the tumour suppressor protein p53 and pRb, respectively. For HPV types 16 and 18, intratypic sequence variation was shown to have biological and clinical significance. The functional significance of sequence variation among HPV 31 variants was studied less intensively. HPV 31 variants belonging to different variant lineages were found to have differences in persistence and in the ability to cause high grade cervical intraepithelial neoplasia. In the present study, we started to explore the functional effects of natural sequence variation of HPV 31 E6 and E7 oncoproteins. The E6 variants were tested for their effects on p53 protein stability and transcriptional activity, while the E7 variants were tested for their effects on pRb protein level and also on the transcriptional activity of E2F transcription factors. HPV 31 E7 variants displayed uniform effects on pRb stability and also on the activity of E2F transcription factors. HPV 31 E6 variants had remarkable differences in the ability to inhibit the trans-activation function of p53 but not in the ability to induce the in vivo degradation of p53. Our results indicate that natural sequence variation of the HPV 31 E6 protein may be involved in the observed differences in the oncogenic potential between HPV 31 variants.

Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalInfection, Genetics and Evolution
Volume43
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

Human papillomavirus 31
Human papillomavirus 16
Papillomaviridae
Oncogene Proteins
Sequence Analysis
phylogenetics
protein
phylogeny
E2F Transcription Factors
cancer
tumor
transcription factors
Tumor Suppressor Protein p53
Human papillomavirus 18
Cervical Intraepithelial Neoplasia
persistence
Protein Stability
Head and Neck Neoplasms
proteins
degradation

Keywords

  • E6
  • E7
  • Functional analysis
  • Human papillomavirus
  • Type 31
  • Variants

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Phylogenetic and functional analysis of sequence variation of human papillomavirus type 31 E6 and E7 oncoproteins. / Ferenczi, Annamária; Gyöngyösi, Eszter; Szalmás, Anita; László, Brigitta; Kónya, József; Veress, G.

In: Infection, Genetics and Evolution, Vol. 43, 01.09.2016, p. 94-100.

Research output: Contribution to journalArticle

Ferenczi, Annamária ; Gyöngyösi, Eszter ; Szalmás, Anita ; László, Brigitta ; Kónya, József ; Veress, G. / Phylogenetic and functional analysis of sequence variation of human papillomavirus type 31 E6 and E7 oncoproteins. In: Infection, Genetics and Evolution. 2016 ; Vol. 43. pp. 94-100.
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