Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) and a predicted structural protein (pSP) of the Chronic bee paralysis virus (CBPV) isolated from various geographic regions

Philippe Blanchard, Frank Schurr, Violaine Olivier, Olivier Celle, Karina Antùnez, T. Bakonyi, Hélène Berthoud, Eric Haubruge, Mariano Higes, Sylwia Kasprzak, Hemma Koeglberger, Per Kryger, Richard Thiéry, Magali Ribière

Research output: Contribution to journalArticle

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Abstract

Chronic bee paralysis virus (CBPV) is responsible for chronic paralysis, an infectious and contagious disease of adult honey bees (Apis mellifera L.). The full-length nucleotide sequences of the two major RNAs of CBPV have previously been characterized. The Orf3 of RNA1 has shown significant similarities to the RNA-dependent RNA polymerase (RdRp) of positive single-stranded RNA viruses, whereas the Orf3 of RNA2 encodes a putative structural protein (pSP). In the present study, honey bees originating from 9 different countries (Austria, Poland, Hungary, Spain, Belgium, Denmark, Switzerland, Uruguay and France) were analysed for the presence of CBPV genome. The complete genomic nucleotide sequence of the RdRp (1947 bp) and of the pSP (543 bp) from 24 honey bee positive samples was determined and the phylogenetic relationship among isolates was investigated. Four distinct genotypes of CBPV were observed.

Original languageEnglish
Pages (from-to)334-338
Number of pages5
JournalVirus Research
Volume144
Issue number1-2
DOIs
Publication statusPublished - Sep 2009

Fingerprint

RNA Replicase
Bees
Paralysis
Viruses
Honey
Proteins
Austria-Hungary
Uruguay
RNA Viruses
Belgium
Poland
Denmark
Switzerland
Spain
France
Communicable Diseases
Genotype
Genome
RNA

Keywords

  • Apis mellifera
  • Chronic bee paralysis virus (CBPV)
  • Phylogenetic analysis
  • Predicted structural protein
  • RNA-dependent RNA polymerase

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) and a predicted structural protein (pSP) of the Chronic bee paralysis virus (CBPV) isolated from various geographic regions. / Blanchard, Philippe; Schurr, Frank; Olivier, Violaine; Celle, Olivier; Antùnez, Karina; Bakonyi, T.; Berthoud, Hélène; Haubruge, Eric; Higes, Mariano; Kasprzak, Sylwia; Koeglberger, Hemma; Kryger, Per; Thiéry, Richard; Ribière, Magali.

In: Virus Research, Vol. 144, No. 1-2, 09.2009, p. 334-338.

Research output: Contribution to journalArticle

Blanchard, P, Schurr, F, Olivier, V, Celle, O, Antùnez, K, Bakonyi, T, Berthoud, H, Haubruge, E, Higes, M, Kasprzak, S, Koeglberger, H, Kryger, P, Thiéry, R & Ribière, M 2009, 'Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) and a predicted structural protein (pSP) of the Chronic bee paralysis virus (CBPV) isolated from various geographic regions', Virus Research, vol. 144, no. 1-2, pp. 334-338. https://doi.org/10.1016/j.virusres.2009.04.025
Blanchard, Philippe ; Schurr, Frank ; Olivier, Violaine ; Celle, Olivier ; Antùnez, Karina ; Bakonyi, T. ; Berthoud, Hélène ; Haubruge, Eric ; Higes, Mariano ; Kasprzak, Sylwia ; Koeglberger, Hemma ; Kryger, Per ; Thiéry, Richard ; Ribière, Magali. / Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) and a predicted structural protein (pSP) of the Chronic bee paralysis virus (CBPV) isolated from various geographic regions. In: Virus Research. 2009 ; Vol. 144, No. 1-2. pp. 334-338.
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