Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant

E. Tóth, A. Vengring, Z. G. Homonnay, Zs Kéki, C. Spröer, A. Borsodi, K. Márialigeti, P. Schumann

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21T exhibited the highest sequence similarity to Bosea minatitlanensis AMX51T (93.43 %) andBosea thiooxidans DSM 9653T (93.36 %); similarity to all other taxa was less than 93.23 %. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18: 1ω7c and 11-methyl C18: 1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21T was 68.9 mol%. A new genus and species,Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21T (= DSM 25521T = NCAIM B 02510T) is the type strain of Phreatobacter oligotrophus.

Original languageEnglish
Pages (from-to)839-845
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 3
DOIs
Publication statusPublished - 2014

Fingerprint

Oligotrophus
water purification
Alphaproteobacteria
Power Plants
Water Purification
alpha-Proteobacteria
power plants
rRNA Genes
power plant
Fatty Acids
fatty acid
lipid
Diaminopimelic Acid
Lipids
Phosphatidylglycerols
Cardiolipins
Water
gene
isoprenoid
new species

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant",
abstract = "Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21T exhibited the highest sequence similarity to Bosea minatitlanensis AMX51T (93.43 {\%}) andBosea thiooxidans DSM 9653T (93.36 {\%}); similarity to all other taxa was less than 93.23 {\%}. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18: 1ω7c and 11-methyl C18: 1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21T was 68.9 mol{\%}. A new genus and species,Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21T (= DSM 25521T = NCAIM B 02510T) is the type strain of Phreatobacter oligotrophus.",
author = "E. T{\'o}th and A. Vengring and Homonnay, {Z. G.} and Zs K{\'e}ki and C. Spr{\"o}er and A. Borsodi and K. M{\'a}rialigeti and P. Schumann",
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T1 - Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant

AU - Tóth, E.

AU - Vengring, A.

AU - Homonnay, Z. G.

AU - Kéki, Zs

AU - Spröer, C.

AU - Borsodi, A.

AU - Márialigeti, K.

AU - Schumann, P.

PY - 2014

Y1 - 2014

N2 - Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21T exhibited the highest sequence similarity to Bosea minatitlanensis AMX51T (93.43 %) andBosea thiooxidans DSM 9653T (93.36 %); similarity to all other taxa was less than 93.23 %. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18: 1ω7c and 11-methyl C18: 1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21T was 68.9 mol%. A new genus and species,Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21T (= DSM 25521T = NCAIM B 02510T) is the type strain of Phreatobacter oligotrophus.

AB - Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21T exhibited the highest sequence similarity to Bosea minatitlanensis AMX51T (93.43 %) andBosea thiooxidans DSM 9653T (93.36 %); similarity to all other taxa was less than 93.23 %. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18: 1ω7c and 11-methyl C18: 1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21T was 68.9 mol%. A new genus and species,Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21T (= DSM 25521T = NCAIM B 02510T) is the type strain of Phreatobacter oligotrophus.

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JO - International Journal of Systematic and Evolutionary Microbiology

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