Photosystem II damage induced by chemically generated singlet oxygen in tobacco leaves

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Abstract

In the present work, we investigated the role of chemically generated singlet oxygen, produced by photodynamic effect of rose bengal, in damaging the PSII complex in tobacco leaves in which protein synthesis-dependent repair was inhibited by infiltration with lincomycin. A 30-min exposure to low-intensity (150 μmol m-2 s-1) photosynthetically active radiation (PAR) induced singlet oxygen production as detected by quenching of 3-[N-(β-diethylaminoethyl)-N-dansyl]aminomethyl-2,2,5,5-tetramethyl-2, 5-dihydro-1H-pyrrole fluorescence in leaves infiltrated with both lincomycin and rose bengal. This light treatment caused photoinhibition of PSII, as revealed by the marked loss both of the photochemical yield and the amount of D1 protein in PSII reaction center. When rose bengal was not present in the leaves, these symptoms of photodamage were not induced by the same low-intensity PAR. However, when excitation pressure on PSII was increased to 1500 μmol m-2 s-1, irreversible photodamage of PSII was also observed, showing that the lincomycin treatment applied in vivo was sufficiently inhibiting protein repair. Our results show that singlet oxygen is able to cause oxidative damage in PSII directly, as suggested earlier and argue against its recently hypothesized role exclusive to inhibiting PSII protein repair (Nishiyama et al. 2006).

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalPhysiologia Plantarum
Volume131
Issue number1
DOIs
Publication statusPublished - Sep 2007

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lincomycin
Singlet Oxygen
singlet oxygen
Photosystem II Protein Complex
Lincomycin
Rose Bengal
photosystem II
Tobacco
tobacco
photosynthetically active radiation
leaves
pyrroles
D1 protein
Proteins
leaf protein
photoinhibition
Radiation
signs and symptoms (plants)
proteins
protein synthesis

ASJC Scopus subject areas

  • Plant Science

Cite this

Photosystem II damage induced by chemically generated singlet oxygen in tobacco leaves. / Hideg, É.; Kós, P.; Vass, I.

In: Physiologia Plantarum, Vol. 131, No. 1, 09.2007, p. 33-40.

Research output: Contribution to journalArticle

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