Photosynthetic functions and thylakoid membrane polypeptide composition in light-sensitive mutants of Chlamydomonas reinhardii

I. Gyurján, A. H. Nagy, G. Frdos, Gy Paless, Á Keresztes, P. Kovacs, Z. Szigeti

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this paper we compared the pigment composition, photochemical activity, chloroplast ultrastructure, thylakoid membrane polypeptide composition and ribosomal content of wild-type and seven light-sensitive mutants of Chlamydomonas reinhardii. All the mutants had low chlorophyll and carotenoid content compared to wild-type. Mutants lts-30 and lts-135 were also characterized by a complete absence of visible carotenoids, while mutant lts-19 was fully deficient in chlorophylls. In most mutants, the chloroplast fragment could not carry out any DCIP photoreduction and O2 evolution was also blocked. The PSI/P700/activity was decreased in most cases. The mutant strains contained mostly single lamellae in their plastids, that is the stacking capacity of the thylakoid membranes was very decreased or fully absent. In most cases the number of lamellae was also very low. The relative amounts of 70 S ribosomes were decreased in all of the mutants. The thylakoid membranes showed anomalies in the region of 24 000-30 000 dalton polypeptides. The common characteristic for them was the relatively higher amount of the 30 000 dalton polypeptide and considerably decreased level of the 27 000 and 24 000 dalton polypeptides relative to the wild-type. These polypeptides were probably constituents of the chlorophyll-protein complex II which has been suggested to be the light harvesting pigment complex for PSII. The polypeptide of 30 000 daltons is the precursor for the LHCP apoprotein (24 000 dalton protein). It may be that the lighstimulated conversion of this precursor into LHCP apoprotein was blocked in our pigment-deficient mutants.

Original languageEnglish
Pages (from-to)255-271
Number of pages17
JournalPhotosynthesis Research
Volume3
Issue number3
DOIs
Publication statusPublished - Jan 1982

Fingerprint

Chlamydomonas reinhardtii
Thylakoids
Chlamydomonas
thylakoids
polypeptides
Membranes
Light
mutants
Peptides
Chemical analysis
Pigments
Apoproteins
Chloroplasts
Carotenoids
Chlorophyll
apoproteins
Chlorophyll Binding Proteins
pigments
Plastids
carotenoids

Keywords

  • Chlamydomonas
  • chloroplast structure
  • membrane polypeptides
  • photosynthesis
  • ribosome

ASJC Scopus subject areas

  • Plant Science

Cite this

Photosynthetic functions and thylakoid membrane polypeptide composition in light-sensitive mutants of Chlamydomonas reinhardii. / Gyurján, I.; Nagy, A. H.; Frdos, G.; Paless, Gy; Keresztes, Á; Kovacs, P.; Szigeti, Z.

In: Photosynthesis Research, Vol. 3, No. 3, 01.1982, p. 255-271.

Research output: Contribution to journalArticle

Gyurján, I. ; Nagy, A. H. ; Frdos, G. ; Paless, Gy ; Keresztes, Á ; Kovacs, P. ; Szigeti, Z. / Photosynthetic functions and thylakoid membrane polypeptide composition in light-sensitive mutants of Chlamydomonas reinhardii. In: Photosynthesis Research. 1982 ; Vol. 3, No. 3. pp. 255-271.
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