Photosynthetic energy conservation investigated by thermoluminescence. Activation energies and half-lives of thermoluminescence bands of chloroplasts determined by mathematical resolution of glow curves

I. Vass, G. Horváth, T. Herczeg, S. Demeter

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Abstract

Thermoluminescence of isolated chloroplasts was analysed by a computer-assisted multicomponent curve fitting procedure to determine the activation energies, the free energies of activation, frequency factors and half-lives of the component bands of the glow curve. Optimal fit was obtained in the temperature region from -80°C to +80°C by the resolution of the glow curve into seven bands with peak positions at -24, -12, +12, +17, +28, +44 and +69°C. All of the activation free energies of the thermoluminescence bands were much higher than 0.59 eV, the minimum free energy of activation required for the back reaction of the primary charge separation as calculated on the basis of the theory of Ross and Calvin (Ross, R.T. and Calvin, M. (1967) Biophys. J. 7, 595-614). The high free energies of activation and long half-lives (longer than 50 ms) of the thermoluminescence bands suggest that thermoluminescence in the temperature region from -80°C to +80°C does not reflect the charge recombination of primary products but represent the reversal of subsequent stabilization steps of the charge separation process which proceed along the acceptor and donor sides of Photosystem II.

Original languageEnglish
Pages (from-to)140-152
Number of pages13
JournalBBA - Bioenergetics
Volume634
Issue numberC
DOIs
Publication statusPublished - 1981

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Thermoluminescence
Chloroplasts
Free energy
Energy conservation
Activation energy
Chemical activation
Temperature
Photosystem II Protein Complex
Genetic Recombination
Curve fitting
Stabilization

Keywords

  • (Chloroplast)
  • Activation energy
  • Energy conservation
  • Photosynthesis
  • Photosystem II
  • Thermoluminescence

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

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title = "Photosynthetic energy conservation investigated by thermoluminescence. Activation energies and half-lives of thermoluminescence bands of chloroplasts determined by mathematical resolution of glow curves",
abstract = "Thermoluminescence of isolated chloroplasts was analysed by a computer-assisted multicomponent curve fitting procedure to determine the activation energies, the free energies of activation, frequency factors and half-lives of the component bands of the glow curve. Optimal fit was obtained in the temperature region from -80°C to +80°C by the resolution of the glow curve into seven bands with peak positions at -24, -12, +12, +17, +28, +44 and +69°C. All of the activation free energies of the thermoluminescence bands were much higher than 0.59 eV, the minimum free energy of activation required for the back reaction of the primary charge separation as calculated on the basis of the theory of Ross and Calvin (Ross, R.T. and Calvin, M. (1967) Biophys. J. 7, 595-614). The high free energies of activation and long half-lives (longer than 50 ms) of the thermoluminescence bands suggest that thermoluminescence in the temperature region from -80°C to +80°C does not reflect the charge recombination of primary products but represent the reversal of subsequent stabilization steps of the charge separation process which proceed along the acceptor and donor sides of Photosystem II.",
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author = "I. Vass and G. Horv{\'a}th and T. Herczeg and S. Demeter",
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T1 - Photosynthetic energy conservation investigated by thermoluminescence. Activation energies and half-lives of thermoluminescence bands of chloroplasts determined by mathematical resolution of glow curves

AU - Vass, I.

AU - Horváth, G.

AU - Herczeg, T.

AU - Demeter, S.

PY - 1981

Y1 - 1981

N2 - Thermoluminescence of isolated chloroplasts was analysed by a computer-assisted multicomponent curve fitting procedure to determine the activation energies, the free energies of activation, frequency factors and half-lives of the component bands of the glow curve. Optimal fit was obtained in the temperature region from -80°C to +80°C by the resolution of the glow curve into seven bands with peak positions at -24, -12, +12, +17, +28, +44 and +69°C. All of the activation free energies of the thermoluminescence bands were much higher than 0.59 eV, the minimum free energy of activation required for the back reaction of the primary charge separation as calculated on the basis of the theory of Ross and Calvin (Ross, R.T. and Calvin, M. (1967) Biophys. J. 7, 595-614). The high free energies of activation and long half-lives (longer than 50 ms) of the thermoluminescence bands suggest that thermoluminescence in the temperature region from -80°C to +80°C does not reflect the charge recombination of primary products but represent the reversal of subsequent stabilization steps of the charge separation process which proceed along the acceptor and donor sides of Photosystem II.

AB - Thermoluminescence of isolated chloroplasts was analysed by a computer-assisted multicomponent curve fitting procedure to determine the activation energies, the free energies of activation, frequency factors and half-lives of the component bands of the glow curve. Optimal fit was obtained in the temperature region from -80°C to +80°C by the resolution of the glow curve into seven bands with peak positions at -24, -12, +12, +17, +28, +44 and +69°C. All of the activation free energies of the thermoluminescence bands were much higher than 0.59 eV, the minimum free energy of activation required for the back reaction of the primary charge separation as calculated on the basis of the theory of Ross and Calvin (Ross, R.T. and Calvin, M. (1967) Biophys. J. 7, 595-614). The high free energies of activation and long half-lives (longer than 50 ms) of the thermoluminescence bands suggest that thermoluminescence in the temperature region from -80°C to +80°C does not reflect the charge recombination of primary products but represent the reversal of subsequent stabilization steps of the charge separation process which proceed along the acceptor and donor sides of Photosystem II.

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KW - Energy conservation

KW - Photosynthesis

KW - Photosystem II

KW - Thermoluminescence

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