Photopigments and photoentrainment in the Syrian golden hamster

Malcolm Von Schantz, Sharleen M. Argamaso-Hernan, A. Szél, Russell G. Foster

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The Syrian golden hamster (Mesocricetus auratus) is an important model in the study of circadian rhythms. However, as in other mammals, little is known about the photoreceptors that mediate circadian entrainment. Using immunocytochemistry and RNA blot hybridization, we found no evidence for the presence of blue- /UV-sensitive opsin. In contrast, green-sensitive cone opsin was demonstrated in the retina both by immunocytochemistry and reverse-transcription PCR. When used as a probe in RNA blot hybridization, this PCR fragment labelled one transcript (5.8 kb) in hamster retinal RNA. These findings are in accordance with preliminary data from other investigators using electroretinography, which showed one cone-mediated photoreceptive mechanism with a maximum sensitivity of 501 nm, but none at shorter wavelengths. However, we found that non-saturating pulses of ultraviolet radiation (357 nm) caused phase shifts in locomotor behaviour. These results corroborate earlier reports that UV radiation can regulate the photoperiodic response in this animal. Having confirmed these apparently contradictory earlier reports, we discuss the mechanisms that might create a UV-triggered non-visual response in a green cone monochromat. Finally, we propose the use of the Syrian golden hamster as a model for photoreceptor development and function in the absence of S/UV cones.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalBrain Research
Volume770
Issue number1-2
DOIs
Publication statusPublished - Oct 3 1997

Fingerprint

Mesocricetus
Cone Opsins
Immunohistochemistry
RNA
Radiation
Opsins
RNA Probes
Electroretinography
Polymerase Chain Reaction
Circadian Rhythm
Cricetinae
Reverse Transcription
Retina
Mammals
Research Personnel

Keywords

  • Circadian entrainment
  • Cone opsin
  • Immunocytochemistry
  • Mesocricetus auratus
  • Phase shift assay
  • RNA
  • Syrian golden hamster

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Photopigments and photoentrainment in the Syrian golden hamster. / Von Schantz, Malcolm; Argamaso-Hernan, Sharleen M.; Szél, A.; Foster, Russell G.

In: Brain Research, Vol. 770, No. 1-2, 03.10.1997, p. 131-138.

Research output: Contribution to journalArticle

Von Schantz, M, Argamaso-Hernan, SM, Szél, A & Foster, RG 1997, 'Photopigments and photoentrainment in the Syrian golden hamster', Brain Research, vol. 770, no. 1-2, pp. 131-138. https://doi.org/10.1016/S0006-8993(97)00791-9
Von Schantz, Malcolm ; Argamaso-Hernan, Sharleen M. ; Szél, A. ; Foster, Russell G. / Photopigments and photoentrainment in the Syrian golden hamster. In: Brain Research. 1997 ; Vol. 770, No. 1-2. pp. 131-138.
@article{7f93a14c9c514c5ea7bed2c433677b8f,
title = "Photopigments and photoentrainment in the Syrian golden hamster",
abstract = "The Syrian golden hamster (Mesocricetus auratus) is an important model in the study of circadian rhythms. However, as in other mammals, little is known about the photoreceptors that mediate circadian entrainment. Using immunocytochemistry and RNA blot hybridization, we found no evidence for the presence of blue- /UV-sensitive opsin. In contrast, green-sensitive cone opsin was demonstrated in the retina both by immunocytochemistry and reverse-transcription PCR. When used as a probe in RNA blot hybridization, this PCR fragment labelled one transcript (5.8 kb) in hamster retinal RNA. These findings are in accordance with preliminary data from other investigators using electroretinography, which showed one cone-mediated photoreceptive mechanism with a maximum sensitivity of 501 nm, but none at shorter wavelengths. However, we found that non-saturating pulses of ultraviolet radiation (357 nm) caused phase shifts in locomotor behaviour. These results corroborate earlier reports that UV radiation can regulate the photoperiodic response in this animal. Having confirmed these apparently contradictory earlier reports, we discuss the mechanisms that might create a UV-triggered non-visual response in a green cone monochromat. Finally, we propose the use of the Syrian golden hamster as a model for photoreceptor development and function in the absence of S/UV cones.",
keywords = "Circadian entrainment, Cone opsin, Immunocytochemistry, Mesocricetus auratus, Phase shift assay, RNA, Syrian golden hamster",
author = "{Von Schantz}, Malcolm and Argamaso-Hernan, {Sharleen M.} and A. Sz{\'e}l and Foster, {Russell G.}",
year = "1997",
month = "10",
day = "3",
doi = "10.1016/S0006-8993(97)00791-9",
language = "English",
volume = "770",
pages = "131--138",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Photopigments and photoentrainment in the Syrian golden hamster

AU - Von Schantz, Malcolm

AU - Argamaso-Hernan, Sharleen M.

AU - Szél, A.

AU - Foster, Russell G.

PY - 1997/10/3

Y1 - 1997/10/3

N2 - The Syrian golden hamster (Mesocricetus auratus) is an important model in the study of circadian rhythms. However, as in other mammals, little is known about the photoreceptors that mediate circadian entrainment. Using immunocytochemistry and RNA blot hybridization, we found no evidence for the presence of blue- /UV-sensitive opsin. In contrast, green-sensitive cone opsin was demonstrated in the retina both by immunocytochemistry and reverse-transcription PCR. When used as a probe in RNA blot hybridization, this PCR fragment labelled one transcript (5.8 kb) in hamster retinal RNA. These findings are in accordance with preliminary data from other investigators using electroretinography, which showed one cone-mediated photoreceptive mechanism with a maximum sensitivity of 501 nm, but none at shorter wavelengths. However, we found that non-saturating pulses of ultraviolet radiation (357 nm) caused phase shifts in locomotor behaviour. These results corroborate earlier reports that UV radiation can regulate the photoperiodic response in this animal. Having confirmed these apparently contradictory earlier reports, we discuss the mechanisms that might create a UV-triggered non-visual response in a green cone monochromat. Finally, we propose the use of the Syrian golden hamster as a model for photoreceptor development and function in the absence of S/UV cones.

AB - The Syrian golden hamster (Mesocricetus auratus) is an important model in the study of circadian rhythms. However, as in other mammals, little is known about the photoreceptors that mediate circadian entrainment. Using immunocytochemistry and RNA blot hybridization, we found no evidence for the presence of blue- /UV-sensitive opsin. In contrast, green-sensitive cone opsin was demonstrated in the retina both by immunocytochemistry and reverse-transcription PCR. When used as a probe in RNA blot hybridization, this PCR fragment labelled one transcript (5.8 kb) in hamster retinal RNA. These findings are in accordance with preliminary data from other investigators using electroretinography, which showed one cone-mediated photoreceptive mechanism with a maximum sensitivity of 501 nm, but none at shorter wavelengths. However, we found that non-saturating pulses of ultraviolet radiation (357 nm) caused phase shifts in locomotor behaviour. These results corroborate earlier reports that UV radiation can regulate the photoperiodic response in this animal. Having confirmed these apparently contradictory earlier reports, we discuss the mechanisms that might create a UV-triggered non-visual response in a green cone monochromat. Finally, we propose the use of the Syrian golden hamster as a model for photoreceptor development and function in the absence of S/UV cones.

KW - Circadian entrainment

KW - Cone opsin

KW - Immunocytochemistry

KW - Mesocricetus auratus

KW - Phase shift assay

KW - RNA

KW - Syrian golden hamster

UR - http://www.scopus.com/inward/record.url?scp=0030853152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030853152&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(97)00791-9

DO - 10.1016/S0006-8993(97)00791-9

M3 - Article

C2 - 9372212

AN - SCOPUS:0030853152

VL - 770

SP - 131

EP - 138

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -