Photoinactivation of Photosystem II by flashing light

András Szilárd, L. Sass, É. Hideg, I. Vass

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Inhibition of Photosystem II (PS II) activity by single turnover visible light flashes was studied in thylakoid membranes isolated form spinach. Flash illumination results in decreased oxygen evolving activity of PS II, which effect is most pronounced when the water-oxidizing complex is in the S 2 and S3 states, and increases with increasing time delay between the subsequent flashes. By applying the fluorescent spin-trap DanePy, we detected the production of singlet oxygen, whose amount was increasing with increasing flash spacing. These findings were explained in the framework of a model, which assumes that recombination of the S2Q B - and S3Q B - states generate the triplet state of the reaction center chlorophyll and lead to the production of singlet oxygen.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalPhotosynthesis Research
Volume84
Issue number1-3
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Singlet Oxygen
singlet oxygen
Photosystem II Protein Complex
photosystem II
Light
Thylakoids
Spinacia oleracea
Chlorophyll
Lighting
spinach
thylakoids
Genetic Recombination
lighting
Time delay
traps
spatial distribution
Oxygen
Membranes
chlorophyll
oxygen

Keywords

  • Low light effect
  • Photoinactivation
  • Photosystem II
  • S-states

ASJC Scopus subject areas

  • Plant Science

Cite this

Photoinactivation of Photosystem II by flashing light. / Szilárd, András; Sass, L.; Hideg, É.; Vass, I.

In: Photosynthesis Research, Vol. 84, No. 1-3, 06.2005, p. 15-20.

Research output: Contribution to journalArticle

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