Porfirin származékok alkalmazása T7 fág fotodinamikus inaktivációjában

A reakció hatékonysága és mechanizmusa

Translated title of the contribution: Photodynamic inactivation of porphyrin sensitized T7 phage: Efficiency and mechanism of action

Marianna Egyeki, Gergely Turóczy, Katalin Tóth, Andrea Fekete, Philippe Maillard, G. Csík

Research output: Contribution to journalArticle

Abstract

We investigated the efficiency and the mechanism of action of two glycoconjugated tetraphenyl porphyrins in their photoreaction with T7 bacteriophage. Both types of porphyrins sensitized the photoinactivation of T7, but the slopes of inactivation kinetics were markedly different. Our result suggests that both type I and type II reaction play a role in the virus inactivation. Optical melting studies revealed structural changes in the protein part but not in the DNA of the photo-chemically treated nucleoprotein complex. Polymerase chain reaction (PCR) analysis failed to demonstrate any DNA damage.

Original languageHungarian
Pages (from-to)97-102
Number of pages6
JournalActa Pharmaceutica Hungarica
Volume73
Issue number2
Publication statusPublished - 2003

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Bacteriophage T7
Porphyrins
Virus Inactivation
Nucleoproteins
Freezing
DNA Damage
Polymerase Chain Reaction
DNA
Proteins

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Porfirin származékok alkalmazása T7 fág fotodinamikus inaktivációjában : A reakció hatékonysága és mechanizmusa. / Egyeki, Marianna; Turóczy, Gergely; Tóth, Katalin; Fekete, Andrea; Maillard, Philippe; Csík, G.

In: Acta Pharmaceutica Hungarica, Vol. 73, No. 2, 2003, p. 97-102.

Research output: Contribution to journalArticle

Egyeki, Marianna ; Turóczy, Gergely ; Tóth, Katalin ; Fekete, Andrea ; Maillard, Philippe ; Csík, G. / Porfirin származékok alkalmazása T7 fág fotodinamikus inaktivációjában : A reakció hatékonysága és mechanizmusa. In: Acta Pharmaceutica Hungarica. 2003 ; Vol. 73, No. 2. pp. 97-102.
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