Photocontrol of subcellular partitioning of phytochrome-B: GFP fusion protein in tobacco seedlings

Gil Patricia, Stefan Kircher, E. Ádám, Erik Bury, L. Kozma-Bognár, Eberhard Schäfer, Ferenc Nagy

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Photomorphogenesis of higher plants is regulated by photoreceptors including the red/far-red light-absorbing phytochromes, blue-UV/A sensing cryptochromes and as yet uncharacterized UV/B receptors. Specific phototransduction pathways that are controlled by either individual or interacting photoreceptors mediate regulation. Phytochrome B (phyB) is the major red light-sensing photoreceptor. Phototransduction mediated by this light sensor has been shown to include light-dependent nuclear import and interaction of phyB with transcription factor-like proteins in the nucleus. Here we report that nuclear import of phyB and physiological responses regulated by this photoreceptor exhibit very similar wavelength- and fluence rate-dependence. Nuclear import of phyB is insensitive to single red, blue and far-red light pulses. It is induced by continuous red light and to a lesser extent by continuous blue light, whereas far-red light is completely ineffective. The data presented indicate that light-dependent partitioning of phyB exhibits features characteristic of blue light responsiveness amplification, a phenomenon that is thought to be mediated by interaction of phyB with CRY1.

Original languageEnglish
Pages (from-to)135-145
Number of pages11
JournalPlant Journal
Volume22
Issue number2
DOIs
Publication statusPublished - Apr 2000

Fingerprint

Phytochrome B
phytochrome
Seedlings
Tobacco
tobacco
Light
seedlings
photoreceptors
far-red light
Proteins
imports
proteins
phototransduction
Cell Nucleus Active Transport
blue light
red light
Light Signal Transduction
photomorphogenesis
Cryptochromes
Phytochrome

ASJC Scopus subject areas

  • Plant Science

Cite this

Photocontrol of subcellular partitioning of phytochrome-B : GFP fusion protein in tobacco seedlings. / Patricia, Gil; Kircher, Stefan; Ádám, E.; Bury, Erik; Kozma-Bognár, L.; Schäfer, Eberhard; Nagy, Ferenc.

In: Plant Journal, Vol. 22, No. 2, 04.2000, p. 135-145.

Research output: Contribution to journalArticle

Patricia, Gil ; Kircher, Stefan ; Ádám, E. ; Bury, Erik ; Kozma-Bognár, L. ; Schäfer, Eberhard ; Nagy, Ferenc. / Photocontrol of subcellular partitioning of phytochrome-B : GFP fusion protein in tobacco seedlings. In: Plant Journal. 2000 ; Vol. 22, No. 2. pp. 135-145.
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