Photochemical reactions of bacteriorhodopsin films

Janos K. Lanyi, G. Váró

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The light-induced transformations of bacteriorhodopsin were followed after laser excitation of thin films with time resolved multichannel spectroscopy. From the measured difference spectra, estimates for the absolute spectra of the intermediates K, L, M, N, and O were produced, and the data were decomposed into sums of individual difference spectra. The latter yield the time-dependent concentrations of the species of the photocycle, from 100 ns to 100 ms. The results show that the kinetics are consistent with a linear reaction sequence containing numerous reversible reactions. The unidirectionality of the charge translocation in this pigment during the photochemical cycle depends on one critical step which had no detectable reverse rate. This step is between the proton release event on one side, and the proton uptake on the other.

Original languageEnglish
Title of host publicationProceedings of the Annual Conference on Engineering in Medicine and Biology
PublisherPubl by IEEE
Pages1714-1715
Number of pages2
Editionpt 4
ISBN (Print)0879425598
Publication statusPublished - 1990
EventProceedings of the 12th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Philadelphia, PA, USA
Duration: Nov 1 1990Nov 4 1990

Other

OtherProceedings of the 12th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CityPhiladelphia, PA, USA
Period11/1/9011/4/90

Fingerprint

Bacteriorhodopsins
Photochemical reactions
Protons
Laser excitation
Pigments
Spectroscopy
Thin films
Kinetics

ASJC Scopus subject areas

  • Bioengineering

Cite this

Lanyi, J. K., & Váró, G. (1990). Photochemical reactions of bacteriorhodopsin films. In Proceedings of the Annual Conference on Engineering in Medicine and Biology (pt 4 ed., pp. 1714-1715). Publ by IEEE.

Photochemical reactions of bacteriorhodopsin films. / Lanyi, Janos K.; Váró, G.

Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 4. ed. Publ by IEEE, 1990. p. 1714-1715.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lanyi, JK & Váró, G 1990, Photochemical reactions of bacteriorhodopsin films. in Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 4 edn, Publ by IEEE, pp. 1714-1715, Proceedings of the 12th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Philadelphia, PA, USA, 11/1/90.
Lanyi JK, Váró G. Photochemical reactions of bacteriorhodopsin films. In Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 4 ed. Publ by IEEE. 1990. p. 1714-1715
Lanyi, Janos K. ; Váró, G. / Photochemical reactions of bacteriorhodopsin films. Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 4. ed. Publ by IEEE, 1990. pp. 1714-1715
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