Photoaffinity labelling with an ATP analog of the N-terminal peptide of myosin

L. Szilagyi, M. Balint, F. A. Sreter, J. Gergely

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Abstract

Photoaffinity labelling of tryptic and chymotryptic heavy meromyosin with 3′O-3-[N-(4-azido-2-nitrophenyl) amino]propionyl-adenosine 5′-triphosphate (arylazido-β-alanine ATP) resulted in incorporation of radioactivity and inhibition of the ATPase activity. ATP prevented the reaction with the photoaffinity label, as shown by the lack of incorporation of 3H and intact ATPase activity. On the tryptic digestion of either type of photoaffinity labeled HMM the label was found in a 25K peptide identifiable with the N-terminus of the myosin heavy chain (Lu et al., Fed. Proc. 37 1695 1978). The results are discussed in the light of previous localization of the reactive thiol groups, SH-1 and SH-2 (Balint et al., Arch. Biochem. Biophys. 190, 793 1978).

Original languageEnglish
Pages (from-to)936-945
Number of pages10
JournalBiochemical and biophysical research communications
Volume87
Issue number3
DOIs
Publication statusPublished - Apr 13 1979

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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