Photoadaptation in Neurospora by Competitive Interaction of Activating and Inhibitory LOV Domains

Erik Malzahn, Stilianos Ciprianidis, K. Káldi, Tobias Schafmeier, Michael Brunner

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Light responses and photoadaptation of Neurospora depend on the photosensory light-oxygen-voltage (LOV) domains of the circadian transcription factor White Collar Complex (WCC) and its negative regulator VIVID (VVD). We found that light triggers LOV-mediated dimerization of the WCC. The activated WCC induces expression of VVD, which then disrupts and inactivates the WCC homodimers by the competitive formation of WCC-VVD heterodimers, leading to photoadaptation. During the day, expression levels of VVD correlate with light intensity, allowing photoadaptation over several orders of magnitude. At night, previously synthesized VVD serves as a molecular memory of the brightness of the preceding day and suppresses responses to light cues of lower intensity. We show that VVD is essential to discriminate between day and night, even in naturally ambiguous photoperiods with moonlight.

Original languageEnglish
Pages (from-to)762-772
Number of pages11
JournalCell
Volume142
Issue number5
DOIs
Publication statusPublished - Sep 3 2010

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Neurospora
Oxygen
Light
Electric potential
Dimerization
Photoperiod
Cues
Luminance
Transcription Factors
Data storage equipment

Keywords

  • Cellbio
  • Signaling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Photoadaptation in Neurospora by Competitive Interaction of Activating and Inhibitory LOV Domains. / Malzahn, Erik; Ciprianidis, Stilianos; Káldi, K.; Schafmeier, Tobias; Brunner, Michael.

In: Cell, Vol. 142, No. 5, 03.09.2010, p. 762-772.

Research output: Contribution to journalArticle

Malzahn, Erik ; Ciprianidis, Stilianos ; Káldi, K. ; Schafmeier, Tobias ; Brunner, Michael. / Photoadaptation in Neurospora by Competitive Interaction of Activating and Inhibitory LOV Domains. In: Cell. 2010 ; Vol. 142, No. 5. pp. 762-772.
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