Photoactivated conformational changes in rhodopsin: A time-resolved spin label study

Zohreh Toossi Farahbakhsh, Kálmán Hideg, Wayne L. Hubbell

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Rhodopsin has been selectively spin-labeled near the cytoplasmic termini of helices C and G. Photoactivation with a light flash induces an electron paramagnetic resonance spectral change in the millisecond time domain, coincident with the appearance of the active metarhodopsin II intermediate. The spectral change is consistent with a small movement near the cytoplasmic termination of the C helix and reverses upon formation of the MIII state. These results provide an important link between the optical changes associated with the retinal chromophore and protein conformational states.

Original languageEnglish
Pages (from-to)1416-1419
Number of pages4
JournalScience
Volume262
Issue number5138
DOIs
Publication statusPublished - Jan 1 1993

ASJC Scopus subject areas

  • General

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