Photoactivated conformational changes in rhodopsin: A time-resolved spin label study

Zohreh Toossi Farahbakhsh, K. Hideg, Wayne L. Hubbell

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Rhodopsin has been selectively spin-labeled near the cytoplasmic termini of helices C and G. Photoactivation with a light flash induces an electron paramagnetic resonance spectra change in the millisecond time domain, coincident with the appearance of the active metarhodopsin II intermediate. The spectral change is consistent with a small movement near the cytoplasmic termination of the C helix and reverses upon formation of the MIII state. These results provide an important link between the optical changes associated with the retinal chromophore and protein conformational states.

Original languageEnglish
Pages (from-to)1416-1419
Number of pages4
JournalScience
Volume262
Issue number5138
Publication statusPublished - Nov 26 1993

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Spin Labels
Rhodopsin
Electron Spin Resonance Spectroscopy
Light
Proteins
metarhodopsins

ASJC Scopus subject areas

  • General

Cite this

Farahbakhsh, Z. T., Hideg, K., & Hubbell, W. L. (1993). Photoactivated conformational changes in rhodopsin: A time-resolved spin label study. Science, 262(5138), 1416-1419.

Photoactivated conformational changes in rhodopsin : A time-resolved spin label study. / Farahbakhsh, Zohreh Toossi; Hideg, K.; Hubbell, Wayne L.

In: Science, Vol. 262, No. 5138, 26.11.1993, p. 1416-1419.

Research output: Contribution to journalArticle

Farahbakhsh, ZT, Hideg, K & Hubbell, WL 1993, 'Photoactivated conformational changes in rhodopsin: A time-resolved spin label study', Science, vol. 262, no. 5138, pp. 1416-1419.
Farahbakhsh, Zohreh Toossi ; Hideg, K. ; Hubbell, Wayne L. / Photoactivated conformational changes in rhodopsin : A time-resolved spin label study. In: Science. 1993 ; Vol. 262, No. 5138. pp. 1416-1419.
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