Phospholipid vesicles increase the survival of freeze-dried human red blood cells

Azadeh Kheirolomoom, Gyana R. Satpathy, Z. Török, Mitali Banerjee, Rachna Bali, Roberta C. Novaes, Erika Little, Danielle M. Manning, Denis M. Dwyre, Fern Tablin, John H. Crowe, Nelly M. Tsvetkova

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In a previous report [Z. Török, G. Satpathy, M. Banerjee, R. Bali, E. Little, R. Novaes, H. Van Ly, D. Dwyre, A. Kheirolomoom, F. Tablin, J.H. Crowe, N.M. Tsvetkova, Preservation of trehalose loaded red blood cells by lyophilization, Cell Preservation Technol. 3 (2005) 96-111.], we presented a method for preserving human red blood cells (RBCs) by loading them with trehalose and then freeze-drying. We have now improved that method, based on the discovery that addition of phospholipid vesicles to the lyophilization buffer substantially reduces hemolysis of freeze-dried RBCs after rehydration. The surviving cells synthesize 2,3-DPG, have low levels of methemoglobin, and have preserved morphology. Among the lipid species we studied, unsaturated PCs were found to be most effective in suppressing hemoglobin leakage. RBC-vesicle interactions depend on vesicle size and structure; unilamellar liposomes with average diameter of less than 300 nm were more effective in reducing the hemolysis than multilamellar vesicles. Trehalose loaded RBCs demonstrated high survival and low levels of methemoglobin during 10 weeks of storage at 4°C in the dry state when lyophilized in the presence of liposomes.

Original languageEnglish
Pages (from-to)290-305
Number of pages16
JournalCryobiology
Volume51
Issue number3
DOIs
Publication statusPublished - Dec 2005

Fingerprint

Phospholipids
phospholipids
Blood
erythrocytes
Erythrocytes
Cells
Trehalose
Freeze Drying
freeze drying
trehalose
Methemoglobin
hemolysis
Hemolysis
2,3-Diphosphoglycerate
Unilamellar Liposomes
Fluid Therapy
rehydration
Liposomes
Cell Communication
Indonesia

Keywords

  • Erythrocytes
  • Freeze-drying
  • Liposomes
  • Long-term stability
  • Preservation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Kheirolomoom, A., Satpathy, G. R., Török, Z., Banerjee, M., Bali, R., Novaes, R. C., ... Tsvetkova, N. M. (2005). Phospholipid vesicles increase the survival of freeze-dried human red blood cells. Cryobiology, 51(3), 290-305. https://doi.org/10.1016/j.cryobiol.2005.08.003

Phospholipid vesicles increase the survival of freeze-dried human red blood cells. / Kheirolomoom, Azadeh; Satpathy, Gyana R.; Török, Z.; Banerjee, Mitali; Bali, Rachna; Novaes, Roberta C.; Little, Erika; Manning, Danielle M.; Dwyre, Denis M.; Tablin, Fern; Crowe, John H.; Tsvetkova, Nelly M.

In: Cryobiology, Vol. 51, No. 3, 12.2005, p. 290-305.

Research output: Contribution to journalArticle

Kheirolomoom, A, Satpathy, GR, Török, Z, Banerjee, M, Bali, R, Novaes, RC, Little, E, Manning, DM, Dwyre, DM, Tablin, F, Crowe, JH & Tsvetkova, NM 2005, 'Phospholipid vesicles increase the survival of freeze-dried human red blood cells', Cryobiology, vol. 51, no. 3, pp. 290-305. https://doi.org/10.1016/j.cryobiol.2005.08.003
Kheirolomoom, Azadeh ; Satpathy, Gyana R. ; Török, Z. ; Banerjee, Mitali ; Bali, Rachna ; Novaes, Roberta C. ; Little, Erika ; Manning, Danielle M. ; Dwyre, Denis M. ; Tablin, Fern ; Crowe, John H. ; Tsvetkova, Nelly M. / Phospholipid vesicles increase the survival of freeze-dried human red blood cells. In: Cryobiology. 2005 ; Vol. 51, No. 3. pp. 290-305.
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