Phospholipase D activity in the Tetrahymena pyriformis GL

P. Kovács, G. Csaba, S. Nakashima, Y. Nozawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Phospholipase D (PLD) is an enzyme which participates in the signalling mechanism cleaving phosphatidylcholine (PC) to choline and phosphatidic acid (PA). In Tetrahymena pyriformis GL this enzyme activity is enhanced by different kinds of agonists (sodium orthovanadate, sodium fluoride and phorbol 12-myristate 13-acetate), and its activity can be inhibited by inhibitors such as pertussis toxin, calphostin C, genistein, trifluoperazine. These results suggest that the PLD signalling pathway is connected with the tyrosine kinase, phospholipase C, phosphatidylinositol and G-protein coupled signalling pathways. By demonstrating the PLD activity in Tetrahymena our knowledge on the signalling mechanisms at a unicellular level has been extended. The results support our view that most transducing mechanisms that are characteristic of mammalian cells are also in the protozoan Tetrahymena.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalCell Biochemistry and Function
Volume15
Issue number1
DOIs
Publication statusPublished - Mar 1997

Fingerprint

Tetrahymena pyriformis
Phospholipase D
Tetrahymena
4 alpha-glucanotransferase
Trifluoperazine
Sodium Fluoride
Phosphatidic Acids
Hyaluronoglucosaminidase
Vanadates
Genistein
Pertussis Toxin
Type C Phospholipases
Phosphatidylinositols
Choline
Phosphatidylcholines
GTP-Binding Proteins
Protein-Tyrosine Kinases
Enzyme activity
Acetates
Sodium

Keywords

  • Evolution
  • Phospholipase D
  • Protozoa
  • Signalling
  • Tetrahymena

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Phospholipase D activity in the Tetrahymena pyriformis GL. / Kovács, P.; Csaba, G.; Nakashima, S.; Nozawa, Y.

In: Cell Biochemistry and Function, Vol. 15, No. 1, 03.1997, p. 53-60.

Research output: Contribution to journalArticle

Kovács, P. ; Csaba, G. ; Nakashima, S. ; Nozawa, Y. / Phospholipase D activity in the Tetrahymena pyriformis GL. In: Cell Biochemistry and Function. 1997 ; Vol. 15, No. 1. pp. 53-60.
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