Phospholipase C and phospholipase A2 are involved in the antiviral activity of human interferon-α

György Premecz, Andrea Markovits, György Bagi, T. Farkas, István Földes

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Treatment of human amniotic cells (UAC) with human interferon-α (Hu-IFNα) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in fatty acid content for the major phospholipids of IFN-treated cells suggest that phospholipases C and A2 are activated by Hu-IFNα . Addition of neomycin (an inhibitor of phospholipase C), as well as mepacrine (an inhibitor of phospholipase A2) to IFN-treated cells inhibited the antiviral activity of Hu-IFNα in the vesicular stomatitis virus (VSV)-UAC system used. These observations indicate that (i) activation of PK-C and (ii) diacylglycerol formation, arachidonic acid and/or lysophosphatidylcholine release are important steps in the mechanism of action of IFN.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalFEBS Letters
Volume249
Issue number2
DOIs
Publication statusPublished - Jun 5 1989

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Phospholipases A2
Type C Phospholipases
Human Activities
Interferons
Antiviral Agents
Cells
Protein Kinase C
Phospholipids
Quinacrine
Lysophosphatidylcholines
Vesicular Stomatitis
Neomycin
Diglycerides
Tetradecanoylphorbol Acetate
Viruses
Arachidonic Acid
Cytosol
Fatty Acids
Chemical activation
Membranes

Keywords

  • Interferon action
  • Mepacrine
  • Neomycin
  • Phospholipase A
  • Phospholipase C
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Phospholipase C and phospholipase A2 are involved in the antiviral activity of human interferon-α. / Premecz, György; Markovits, Andrea; Bagi, György; Farkas, T.; Földes, István.

In: FEBS Letters, Vol. 249, No. 2, 05.06.1989, p. 257-260.

Research output: Contribution to journalArticle

Premecz, György ; Markovits, Andrea ; Bagi, György ; Farkas, T. ; Földes, István. / Phospholipase C and phospholipase A2 are involved in the antiviral activity of human interferon-α. In: FEBS Letters. 1989 ; Vol. 249, No. 2. pp. 257-260.
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