Phosphoinositide substrates of myotubularin affect voltage-activated Ca2+ release in skeletal muscle

Estela González Rodríguez, Romain Lefebvre, Dóra Bodnár, Claude Legrand, P. Szentesi, János Vincze, Karine Poulard, Justine Bertrand-Michel, L. Csernoch, Anna Buj-Bello, Vincent Jacquemond

Research output: Contribution to journalArticle

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Abstract

Skeletal muscle excitation-contraction (E-C) coupling is altered in several models of phosphatidylinositol phosphate (PtdInsP) phosphatase deficiency and ryanodine receptor activity measured in vitro was reported to be affected by certain PtdInsPs, thus prompting investigation of the physiological role of PtdInsPs in E-C coupling. We measured intracellular Ca2+ transients in voltage-clamped mouse muscle fibres microinjected with a solution containing a PtdInsP substrate (PtdIns(3,5)P 2 or PtdIns(3)P) or product (PtdIns(5)P or PtdIns) of the myotubularin phosphatase MTM1. No significant change was observed in the presence of either PtdIns(5)P or PtdIns but peak SR Ca2+ release was depressed by ~30% and 50% in fibres injected with PtdIns(3,5)P 2 and PtdIns(3)P, respectively, with no concurrent alteration in the membrane current signals associated with the DHPR function as well as in the voltage dependence of Ca2+ release inactivation. In permeabilized muscle fibres, the frequency of spontaneous Ca2+ release events was depressed in the presence of the three tested phosphorylated forms of PtdInsP with PtdIns(3,5)P 2 being the most effective, leading to an almost complete disappearance of Ca2+ release events. Results support the possibility that pathological accumulation of MTM1 substrates may acutely depress ryanodine receptor-mediated Ca2+ release. Overexpression of a mCherry-tagged form of MTM1 in muscle fibres revealed a striated pattern consistent with the triadic area. Ca2+ release remained although unaffected by MTM1 overexpression and was also unaffected by the PtdIns-3-kinase inhibitor LY2940002, suggesting that the 3-phosphorylated PtdIns lipids active on voltage-activated Ca2+ release are inherently maintained at a low level, inefficient on Ca2+ release in normal conditions.

Original languageEnglish
Pages (from-to)973-985
Number of pages13
JournalPflugers Archiv European Journal of Physiology
Volume466
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Phosphatidylinositols
Muscle
Skeletal Muscle
Phosphatidylinositol Phosphates
Electric potential
Substrates
Excitation Contraction Coupling
Ryanodine Receptor Calcium Release Channel
Phosphoric Monoester Hydrolases
Muscles
Fibers
Muscle Contraction
Phosphatidylinositol 3-Kinases
myotubularin
phosphatidylinositol 5-phosphate
Lipids
Membranes

Keywords

  • Calcium homeostasis
  • Excitation-contraction coupling
  • Phosphatidylinositol phosphate
  • Ryanodine receptor

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)
  • Medicine(all)

Cite this

Phosphoinositide substrates of myotubularin affect voltage-activated Ca2+ release in skeletal muscle. / González Rodríguez, Estela; Lefebvre, Romain; Bodnár, Dóra; Legrand, Claude; Szentesi, P.; Vincze, János; Poulard, Karine; Bertrand-Michel, Justine; Csernoch, L.; Buj-Bello, Anna; Jacquemond, Vincent.

In: Pflugers Archiv European Journal of Physiology, Vol. 466, No. 5, 2014, p. 973-985.

Research output: Contribution to journalArticle

González Rodríguez, E, Lefebvre, R, Bodnár, D, Legrand, C, Szentesi, P, Vincze, J, Poulard, K, Bertrand-Michel, J, Csernoch, L, Buj-Bello, A & Jacquemond, V 2014, 'Phosphoinositide substrates of myotubularin affect voltage-activated Ca2+ release in skeletal muscle', Pflugers Archiv European Journal of Physiology, vol. 466, no. 5, pp. 973-985. https://doi.org/10.1007/s00424-013-1346-5
González Rodríguez, Estela ; Lefebvre, Romain ; Bodnár, Dóra ; Legrand, Claude ; Szentesi, P. ; Vincze, János ; Poulard, Karine ; Bertrand-Michel, Justine ; Csernoch, L. ; Buj-Bello, Anna ; Jacquemond, Vincent. / Phosphoinositide substrates of myotubularin affect voltage-activated Ca2+ release in skeletal muscle. In: Pflugers Archiv European Journal of Physiology. 2014 ; Vol. 466, No. 5. pp. 973-985.
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AU - Vincze, János

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