Phosphatidylcholine could be the source of 1,2-DAG which activates protein kinase C in EGF-stimulated colon carcinoma cells (HT29)

Agnes Balogh, O. Csuka, István Teplán, G. Kéri

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In our previous study (A. Balogh et al, Cell. Signalling 5 (6), 795-802, 1993.), we have shown that epidermal growth factor (EGF) increased protein kinase C (PKC) activities in colon carcinoma cell line (HT29), possibly through the increased 1,2-diacylglycerol (1,2-DAG) production via phosphatidylcholine (PC). Here we investigate the effect of the well-known PKC activator 12-O-tetradecanoyl-2 phorbol-13-acetate (TPA), on the levels of 32P incorporation into EGF induced phosphatidylinositols (PI, PI4P, PI4, 5P2) and different phospholipids (PC, PA, PS) as well as on induced tyrosine kinase activity. TPA significantly decreased the effects of EGF and it had the biggest inhibitory effect on EGF induced PC level. These data support our contention that PC plays an important role in the activation of PKC via 1,2-DAG production in the EGF stimulated pathway.

Original languageEnglish
Pages (from-to)793-801
Number of pages9
JournalCellular Signalling
Volume7
Issue number8
DOIs
Publication statusPublished - 1995

Fingerprint

HT29 Cells
Phosphatidylcholines
Epidermal Growth Factor
Protein Kinase C
Colon
Carcinoma
Tetradecanoylphorbol Acetate
Phosphatidylinositols
Protein-Tyrosine Kinases
Phospholipids
Acetates
1,2-diacylglycerol
Cell Line

Keywords

  • 12-O-tetradecanoylphorbol-13-acetate
  • Colon carcinoma
  • Epidermal growth factor
  • Phosphatidylcholine
  • Phosphatidylinositols
  • Protein kinase C
  • Tyrosine kinase activity

ASJC Scopus subject areas

  • Cell Biology

Cite this

Phosphatidylcholine could be the source of 1,2-DAG which activates protein kinase C in EGF-stimulated colon carcinoma cells (HT29). / Balogh, Agnes; Csuka, O.; Teplán, István; Kéri, G.

In: Cellular Signalling, Vol. 7, No. 8, 1995, p. 793-801.

Research output: Contribution to journalArticle

@article{d753ec5f79244c4095e6878567715889,
title = "Phosphatidylcholine could be the source of 1,2-DAG which activates protein kinase C in EGF-stimulated colon carcinoma cells (HT29)",
abstract = "In our previous study (A. Balogh et al, Cell. Signalling 5 (6), 795-802, 1993.), we have shown that epidermal growth factor (EGF) increased protein kinase C (PKC) activities in colon carcinoma cell line (HT29), possibly through the increased 1,2-diacylglycerol (1,2-DAG) production via phosphatidylcholine (PC). Here we investigate the effect of the well-known PKC activator 12-O-tetradecanoyl-2 phorbol-13-acetate (TPA), on the levels of 32P incorporation into EGF induced phosphatidylinositols (PI, PI4P, PI4, 5P2) and different phospholipids (PC, PA, PS) as well as on induced tyrosine kinase activity. TPA significantly decreased the effects of EGF and it had the biggest inhibitory effect on EGF induced PC level. These data support our contention that PC plays an important role in the activation of PKC via 1,2-DAG production in the EGF stimulated pathway.",
keywords = "12-O-tetradecanoylphorbol-13-acetate, Colon carcinoma, Epidermal growth factor, Phosphatidylcholine, Phosphatidylinositols, Protein kinase C, Tyrosine kinase activity",
author = "Agnes Balogh and O. Csuka and Istv{\'a}n Tepl{\'a}n and G. K{\'e}ri",
year = "1995",
doi = "10.1016/0898-6568(95)02007-1",
language = "English",
volume = "7",
pages = "793--801",
journal = "Cellular Signalling",
issn = "0898-6568",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Phosphatidylcholine could be the source of 1,2-DAG which activates protein kinase C in EGF-stimulated colon carcinoma cells (HT29)

AU - Balogh, Agnes

AU - Csuka, O.

AU - Teplán, István

AU - Kéri, G.

PY - 1995

Y1 - 1995

N2 - In our previous study (A. Balogh et al, Cell. Signalling 5 (6), 795-802, 1993.), we have shown that epidermal growth factor (EGF) increased protein kinase C (PKC) activities in colon carcinoma cell line (HT29), possibly through the increased 1,2-diacylglycerol (1,2-DAG) production via phosphatidylcholine (PC). Here we investigate the effect of the well-known PKC activator 12-O-tetradecanoyl-2 phorbol-13-acetate (TPA), on the levels of 32P incorporation into EGF induced phosphatidylinositols (PI, PI4P, PI4, 5P2) and different phospholipids (PC, PA, PS) as well as on induced tyrosine kinase activity. TPA significantly decreased the effects of EGF and it had the biggest inhibitory effect on EGF induced PC level. These data support our contention that PC plays an important role in the activation of PKC via 1,2-DAG production in the EGF stimulated pathway.

AB - In our previous study (A. Balogh et al, Cell. Signalling 5 (6), 795-802, 1993.), we have shown that epidermal growth factor (EGF) increased protein kinase C (PKC) activities in colon carcinoma cell line (HT29), possibly through the increased 1,2-diacylglycerol (1,2-DAG) production via phosphatidylcholine (PC). Here we investigate the effect of the well-known PKC activator 12-O-tetradecanoyl-2 phorbol-13-acetate (TPA), on the levels of 32P incorporation into EGF induced phosphatidylinositols (PI, PI4P, PI4, 5P2) and different phospholipids (PC, PA, PS) as well as on induced tyrosine kinase activity. TPA significantly decreased the effects of EGF and it had the biggest inhibitory effect on EGF induced PC level. These data support our contention that PC plays an important role in the activation of PKC via 1,2-DAG production in the EGF stimulated pathway.

KW - 12-O-tetradecanoylphorbol-13-acetate

KW - Colon carcinoma

KW - Epidermal growth factor

KW - Phosphatidylcholine

KW - Phosphatidylinositols

KW - Protein kinase C

KW - Tyrosine kinase activity

UR - http://www.scopus.com/inward/record.url?scp=0028785612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028785612&partnerID=8YFLogxK

U2 - 10.1016/0898-6568(95)02007-1

DO - 10.1016/0898-6568(95)02007-1

M3 - Article

C2 - 8593248

AN - SCOPUS:0028785612

VL - 7

SP - 793

EP - 801

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 8

ER -