Phosphatidylcholine could be the source of 1,2-DAG which activates protein kinase C in EGF-stimulated colon carcinoma cells (HT29)

Agnes Balogh, Orsolya Csuka, István Teplán, György Kéri

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11 Citations (Scopus)

Abstract

In our previous study (A. Balogh et al, Cell. Signalling 5 (6), 795-802, 1993.), we have shown that epidermal growth factor (EGF) increased protein kinase C (PKC) activities in colon carcinoma cell line (HT29), possibly through the increased 1,2-diacylglycerol (1,2-DAG) production via phosphatidylcholine (PC). Here we investigate the effect of the well-known PKC activator 12-O-tetradecanoyl-2 phorbol-13-acetate (TPA), on the levels of 32P incorporation into EGF induced phosphatidylinositols (PI, PI4P, PI4, 5P2) and different phospholipids (PC, PA, PS) as well as on induced tyrosine kinase activity. TPA significantly decreased the effects of EGF and it had the biggest inhibitory effect on EGF induced PC level. These data support our contention that PC plays an important role in the activation of PKC via 1,2-DAG production in the EGF stimulated pathway.

Original languageEnglish
Pages (from-to)793-801
Number of pages9
JournalCellular Signalling
Volume7
Issue number8
DOIs
Publication statusPublished - Nov 1995

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Keywords

  • 12-O-tetradecanoylphorbol-13-acetate
  • Colon carcinoma
  • Epidermal growth factor
  • Phosphatidylcholine
  • Phosphatidylinositols
  • Protein kinase C
  • Tyrosine kinase activity

ASJC Scopus subject areas

  • Cell Biology

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