Phosphate transport, membrane potential, and movements of calcium in rat liver mitochondria

E. Ligeti, Gergely L. Lukács

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The membrane potential and calcium accumulation of mitochondria were followed by ion-specific electrodes in the presence of the proton-donor anions phosphate, acetate, glutamate, and beta-hydroxybutyrate. Phosphate was the only anion which allowed rapid and complete restoration of both the membrane potential and the steady-state extramitochondrial calcium concentration after the uptake of 100-200 nmol calcium per mg protein. If there was no influx of any proton-donor anion, the extent of calcium uptake depended on the intramitochondrial phosphate content. Both the fall of the membrane potential and the increase of the external calcium concentration brought about by a given amount of uncoupler were counteracted by phosphate transported into the mitochondria.

Original languageEnglish
Pages (from-to)101-113
Number of pages13
JournalJournal of Bioenergetics and Biomembranes
Volume16
Issue number2
DOIs
Publication statusPublished - Apr 1984

Fingerprint

Liver Mitochondrion
Membrane Potentials
Phosphates
Calcium
Anions
Protons
Mitochondria
3-Hydroxybutyric Acid
Glutamic Acid
Electrodes
Acetates
Ions
Proteins

Keywords

  • calcium
  • liver
  • membrane potential
  • Mitochondria
  • phosphate
  • transport
  • uncoupler

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Phosphate transport, membrane potential, and movements of calcium in rat liver mitochondria. / Ligeti, E.; Lukács, Gergely L.

In: Journal of Bioenergetics and Biomembranes, Vol. 16, No. 2, 04.1984, p. 101-113.

Research output: Contribution to journalArticle

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