Phosphatase induction in roots of winter wheat during adaptation to phosphorus deficiency

Andrea Szabó‐Nagy, Zoltán Oláh, László Erdei

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of phosphate supply during the first 4 weeks of the life cycle of wheat (Triticum aestivum L. cv. Martonvásári‐8) was investigated by following growth of seedlings, P levels in roots and shoots, changes of soluble phosphatases in roots and alteration of Ca2+ ‐ and Mg2+‐ATPase activity in the microsomal fraction. Plants were grown in complete nutrient solution supplemented with different levels of phosphate. Maximal growth rate was attained at 0.2 mM phosphate. The total P level in plants increased with increasing phosphate concentration in the growth solution, however, it decreased with age. Microsomal ATPase activity in 14‐day‐old plants increased with phosphorus deficiency. Using phosphocellulose column chromatography, a phosphatase (EC 3.1.3.2) induced by phosphorus deficiency was purified and partially characterized from the 30 000 g supernatant from roots of 14‐ to 30‐day‐old wheat plants. Na‐pyrophosphate, p‐nitrophenylphosphate, ATP, ADP, AMP, O‐phosphoryl‐l‐serine and glucose‐6‐phosphate were all substrates for the enzyme. Its native molecular weight was 42 kDa as determined by Sephadex G‐200 column chromatography. Readdition of phosphate to the growth solution resulted in a gradual decrease of the phosphatase activity, probably due to repression of its synthesis. We hypothesize that the extra phosphatase may participate in the adaptation mechanism under phosphorus‐deficient conditions.

Original languageEnglish
Pages (from-to)544-552
Number of pages9
JournalPhysiologia Plantarum
Volume70
Issue number3
DOIs
Publication statusPublished - Jan 1 1987

Fingerprint

Phosphoric Monoester Hydrolases
Phosphorus
Triticum
winter wheat
Phosphates
phosphates
phosphorus
Growth
adenosinetriphosphatase
Chromatography
chromatography
Ca(2+) Mg(2+)-ATPase
wheat
enzyme substrates
Adenosine Monophosphate
Life Cycle Stages
Seedlings
acid phosphatase
Adenosine Diphosphate
seedling growth

Keywords

  • Acid phosphatase
  • ATPase
  • phosphate
  • shoot to root ratio
  • Triticum aestivum

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Phosphatase induction in roots of winter wheat during adaptation to phosphorus deficiency. / Szabó‐Nagy, Andrea; Oláh, Zoltán; Erdei, László.

In: Physiologia Plantarum, Vol. 70, No. 3, 01.01.1987, p. 544-552.

Research output: Contribution to journalArticle

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