Phenotypic characterization and molecular taxonomic studies on Bacillus and related isolates from Phragmites australis periphyton

A. Borsodi, Judit Makk, Anna Rusznyák, Balázs Vajna, Györgyi Taba, K. Márialigeti

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Reeds may play important role in the self-purification of aquatic habitats due to the filtration capacity and their periphyton communities developing on the underwater plant surfaces. The efficiency of this process and the transformation of organic substances can be influenced by the species composition and activity of microbial communities, including Bacillus and related species. For cultivation based bacteriological examinations reed periphyton samples were collected from 30 cm beneath the water surface of Lake Velencei and the Soroksár Danube branch (Hungary). After a primary selection 40 Bacillus and related strains were investigated by traditional morphological, biochemical tests, API 20E, API 50CHB and BIOLOG GP2 systems, and identified by 16S rDNA sequence comparison. The isolated strains were characterized by wide biochemical activity spectrum (i.e., the metabolism of carbohydrates and biopolymers) as well as widespread ecological tolerance based on their NaCl and pH range investigations. Species with facultative alkaliphilic features (Marinibacillus marinus, Bacillus firmus) were detected only from the reed biofilm of Lake Velencei, while alkalitolerant Bacillus and related species from both sampling sites. 22 endospore-forming strains were identified as members of species B. cereus, B. firmus, B. flexus, B. licheniformis, B. megaterium, B. muralis, B. pumilus, B. subtilis and Marinibacillus marinus. Only one species, with 95-96% sequence similarities to B. pumilus was found to be common among the strains from Lake Velencei and the Soroksár Danube branch. Altogether, 18 strains could not be identified as known species of Bacillus, Brevibacillus and Paenibacillus, hence they may represent new bacterial taxa.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalAquatic Botany
Volume86
Issue number3
DOIs
Publication statusPublished - Apr 2007

Fingerprint

periphyton
Phragmites australis
Bacillus (bacteria)
Bacillus firmus
lakes
Bacillus flexus
Brevibacillus
Paenibacillus
endospores
lake
aquatic habitat
biopolymers
carbohydrate metabolism
Bacillus cereus
Hungary
biofilm
self purification
microbial communities
organic matter
sampling

Keywords

  • API
  • Bacillus
  • BIOLOG
  • Periphyton
  • Phragmites australis

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Phenotypic characterization and molecular taxonomic studies on Bacillus and related isolates from Phragmites australis periphyton. / Borsodi, A.; Makk, Judit; Rusznyák, Anna; Vajna, Balázs; Taba, Györgyi; Márialigeti, K.

In: Aquatic Botany, Vol. 86, No. 3, 04.2007, p. 243-252.

Research output: Contribution to journalArticle

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abstract = "Reeds may play important role in the self-purification of aquatic habitats due to the filtration capacity and their periphyton communities developing on the underwater plant surfaces. The efficiency of this process and the transformation of organic substances can be influenced by the species composition and activity of microbial communities, including Bacillus and related species. For cultivation based bacteriological examinations reed periphyton samples were collected from 30 cm beneath the water surface of Lake Velencei and the Soroks{\'a}r Danube branch (Hungary). After a primary selection 40 Bacillus and related strains were investigated by traditional morphological, biochemical tests, API 20E, API 50CHB and BIOLOG GP2 systems, and identified by 16S rDNA sequence comparison. The isolated strains were characterized by wide biochemical activity spectrum (i.e., the metabolism of carbohydrates and biopolymers) as well as widespread ecological tolerance based on their NaCl and pH range investigations. Species with facultative alkaliphilic features (Marinibacillus marinus, Bacillus firmus) were detected only from the reed biofilm of Lake Velencei, while alkalitolerant Bacillus and related species from both sampling sites. 22 endospore-forming strains were identified as members of species B. cereus, B. firmus, B. flexus, B. licheniformis, B. megaterium, B. muralis, B. pumilus, B. subtilis and Marinibacillus marinus. Only one species, with 95-96{\%} sequence similarities to B. pumilus was found to be common among the strains from Lake Velencei and the Soroks{\'a}r Danube branch. Altogether, 18 strains could not be identified as known species of Bacillus, Brevibacillus and Paenibacillus, hence they may represent new bacterial taxa.",
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