Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and enteroaggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya

M. A. Dow, I. Tóth, A. Malik, M. Herpay, N. Nógrády, K. S. Ghenghesh, B. Nagy

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (enteroaggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae+, EAF+, bfp+) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an enteroaggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30% harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC.

Original languageEnglish
Pages (from-to)100-113
Number of pages14
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume29
Issue number2-3
DOIs
Publication statusPublished - Mar 1 2006

Keywords

  • Adhesion
  • Antibiotic resistance
  • Diarrhoea
  • EAEC
  • EPEC
  • HeLa
  • Integrons
  • Libia

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

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