Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and enteroaggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya

M. A. Dow, I. Tóth, A. Malik, M. Herpay, N. Nógrády, K. S. Ghenghesh, B. Nagy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (enteroaggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae+, EAF+, bfp+) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an enteroaggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30% harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC.

Original languageEnglish
Pages (from-to)100-113
Number of pages14
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume29
Issue number2-3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Libya
Enteropathogenic Escherichia coli
enteropathogenic Escherichia coli
Escherichia coli
Integrons
Northern Africa
Genetic Heterogeneity
Microbial Drug Resistance
HeLa Cells
Virulence
Diarrhea
Anti-Bacterial Agents
pathotypes
antibiotic resistance
diarrhea
serotypes
virulence
antibiotics

Keywords

  • Adhesion
  • Antibiotic resistance
  • Diarrhoea
  • EAEC
  • EPEC
  • HeLa
  • Integrons
  • Libia

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • Microbiology
  • veterinary(all)

Cite this

@article{552b502e091548d1a5469291e4346bae,
title = "Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and enteroaggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya",
abstract = "A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (enteroaggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae+, EAF+, bfp+) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an enteroaggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30{\%} harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC.",
keywords = "Adhesion, Antibiotic resistance, Diarrhoea, EAEC, EPEC, HeLa, Integrons, Libia",
author = "Dow, {M. A.} and I. T{\'o}th and A. Malik and M. Herpay and N. N{\'o}gr{\'a}dy and Ghenghesh, {K. S.} and B. Nagy",
year = "2006",
month = "3",
doi = "10.1016/j.cimid.2006.01.005",
language = "English",
volume = "29",
pages = "100--113",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
issn = "0147-9571",
publisher = "Elsevier Limited",
number = "2-3",

}

TY - JOUR

T1 - Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and enteroaggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya

AU - Dow, M. A.

AU - Tóth, I.

AU - Malik, A.

AU - Herpay, M.

AU - Nógrády, N.

AU - Ghenghesh, K. S.

AU - Nagy, B.

PY - 2006/3

Y1 - 2006/3

N2 - A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (enteroaggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae+, EAF+, bfp+) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an enteroaggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30% harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC.

AB - A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (enteroaggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae+, EAF+, bfp+) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an enteroaggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30% harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC.

KW - Adhesion

KW - Antibiotic resistance

KW - Diarrhoea

KW - EAEC

KW - EPEC

KW - HeLa

KW - Integrons

KW - Libia

UR - http://www.scopus.com/inward/record.url?scp=33646348700&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646348700&partnerID=8YFLogxK

U2 - 10.1016/j.cimid.2006.01.005

DO - 10.1016/j.cimid.2006.01.005

M3 - Article

VL - 29

SP - 100

EP - 113

JO - Comparative Immunology, Microbiology and Infectious Diseases

JF - Comparative Immunology, Microbiology and Infectious Diseases

SN - 0147-9571

IS - 2-3

ER -