Phenotypic and genetic characterisation of methicillin-resistant Staphylococcus aureus strains isolated from the university hospitals of Debrecen

J. Szabó, Z. Dombrádi, O. Dobay, P. Orosi, J. Kónya, K. Nagy, F. Rozgonyi

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7 Citations (Scopus)

Abstract

The purpose of this study was to characterise methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 2005 at the university hospitals of Debrecen, Hungary. Three hundred and thirty-nine MRSA strains were isolated from 102 patients at 18 different clinics. Their sensitivity to oxacillin and ten other antibiotics was determined. For genotypic analysis, phage typing and pulsed-field gel electrophoresis (PFGE) was performed. The rate of MRSA strains increased to 7.2% in 2005, especially at the clinics of surgery, pulmonology and paediatrics. No vancomycin- or teicoplanin-resistant strains were found. The resistance to erythromycin, clindamycin and ciprofloxacin was nearly 100% and multi-resistance was very frequent. Fifty-eight percent of the isolates belonged to mixed phage types and 8% was non-typable. One PFGE clone contained 58.2% of all strains and two further major clones were found at a separately located clinical block, indicating intra-hospital spread. We can conclude that MRSA exhibits an increasing nosocomial problem also in Hungary.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Hungary
Pulsed Field Gel Electrophoresis
Clone Cells
Bacteriophage Typing
Teicoplanin
Oxacillin
Pulmonary Medicine
Clindamycin
Erythromycin
Vancomycin
Ciprofloxacin
Bacteriophages
Pediatrics
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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abstract = "The purpose of this study was to characterise methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 2005 at the university hospitals of Debrecen, Hungary. Three hundred and thirty-nine MRSA strains were isolated from 102 patients at 18 different clinics. Their sensitivity to oxacillin and ten other antibiotics was determined. For genotypic analysis, phage typing and pulsed-field gel electrophoresis (PFGE) was performed. The rate of MRSA strains increased to 7.2{\%} in 2005, especially at the clinics of surgery, pulmonology and paediatrics. No vancomycin- or teicoplanin-resistant strains were found. The resistance to erythromycin, clindamycin and ciprofloxacin was nearly 100{\%} and multi-resistance was very frequent. Fifty-eight percent of the isolates belonged to mixed phage types and 8{\%} was non-typable. One PFGE clone contained 58.2{\%} of all strains and two further major clones were found at a separately located clinical block, indicating intra-hospital spread. We can conclude that MRSA exhibits an increasing nosocomial problem also in Hungary.",
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AU - Dombrádi, Z.

AU - Dobay, O.

AU - Orosi, P.

AU - Kónya, J.

AU - Nagy, K.

AU - Rozgonyi, F.

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AB - The purpose of this study was to characterise methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 2005 at the university hospitals of Debrecen, Hungary. Three hundred and thirty-nine MRSA strains were isolated from 102 patients at 18 different clinics. Their sensitivity to oxacillin and ten other antibiotics was determined. For genotypic analysis, phage typing and pulsed-field gel electrophoresis (PFGE) was performed. The rate of MRSA strains increased to 7.2% in 2005, especially at the clinics of surgery, pulmonology and paediatrics. No vancomycin- or teicoplanin-resistant strains were found. The resistance to erythromycin, clindamycin and ciprofloxacin was nearly 100% and multi-resistance was very frequent. Fifty-eight percent of the isolates belonged to mixed phage types and 8% was non-typable. One PFGE clone contained 58.2% of all strains and two further major clones were found at a separately located clinical block, indicating intra-hospital spread. We can conclude that MRSA exhibits an increasing nosocomial problem also in Hungary.

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