Phenomenological approach to the mechanism of retention in RP HPLC

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The retention process in reversed-phase liquid chromatography (RPLC) and the mechanism behind it can best be followed by a phenomenological description of molecular interactions. This can be accomplished by using the linear salvation-energy relationship (LSER). The retention factor (log k) can be expressed as the difference between the sum of the positive, retention-increasing, terms and the sum of the negative, retention-reducing, terms of the LSER equation. Calculated and measured log k values were compared for five columns with widely different characteristics. From among the 32 test solutes used ten with significantly different salvation properties were selected to demonstrate the effect of the molecular properties of the solutes on the mechanism of retention. The effect of mobile-phase composition on the retention process was also demonstrated. The relative contributions of the different molecular interactions to the mechanism of retention was illustrated by comparing the percentage contributions of the individual terms of the LSER equation.

Original languageEnglish
Pages (from-to)S31-S39
Issue numberSUPPL.
Publication statusPublished - Jul 25 2002



  • Column liquid chromatography
  • Effect of molecular properties of the solutes
  • Linear salvation-energy relationships
  • Molecular interactions

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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