Plum rootstocks are usually propagated by hardwood cuttings, but there is seasonal variability in rooting potential during the dormant season. These changes are well known in some species; the maximal rooting occurs at the beginning and at the end of the dormant season, in the intervening period the rooting is minimal. It can be argued that this seasonality might be caused by root-promoting factors, that are in some way related to the activity of certain enzymes, such as peroxidase and polyphenol oxidase, or with phenolic content. In a series of trials the rooting potential, peroxidase, polyphenol oxidase activity, phenolic content and bud dormancy were followed, looking for the possible links between rooting and these components. In our experiments, rooting percentages were higher in October and February, and were minimal in January, which confirms the data from previous literature that shows that seasonal changes in rooting potential exist in plum rootstocks. The content of phenolic compounds had a seasonal fluctuation as well; starting from a relatively low level in September, reaching a peak in November, decreased until the middle of December, followed by a peak again at the end of January, and finally decreased in February. The activity of polyphenol oxidase also showed a seasonal pattern. The phenolic content of buds and rooting of Marianna GF 8-1 had linear correlation. These components are seem to be correlated with dormancy. Both the beginning and the end of endodormancy seem to be associated with increased PPO activity.