Pheno- and genotypic features of epstein-barr virus associated B-cell lymphoproliferations in peripheral t-cell lymphomas

Gábor Smuk, A. Illés, Katalin Keresztes, L. Kereskai, Balázs Márton, Zsófia Nagy, Ágnes Lacza, László Pajor

Research output: Contribution to journalArticle

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Abstract

Among the 300 peripheral T-cell lymphomas (PTCL) searched for EBV positive non-resting B-cells by EBER in situ hybridization 12 have been identified with various forms of EBV-driven B-cell proliferation. This could be categorized into three major forms. i. In the first form scattered immature, mononuclear B-cells of immuno-, centroblastic type with CD20+. CD30+ CD45+, LMP1+ phenotype, reactive appearance and polyclonal immunoglobulin heavy chains gene rearrangement (IgH-R) were admixed to the PTCL cells. ii. The second form mimicked diffuse large B-cell lymphoma as homogenous sheets, largely demarcated from the PTCL, of mononuclear, immature B-cell of CD20+, CD30+, CD45+, LMP1+, EBNA-2+ phenotype but with lack of monoclonal IgH-R were present. iii. In the third form scattered Hodgkin-Reed-Sternberg (HRS) type of cells were noticed which exhibited the CD15+/-, CD20-/+, CD30+, CD45-, LMP1+, EBNA-2- phenotype and in 50% showed clonal IgH gene rearrangement in whole tissue DNA extract. The IgH associated transcription factors' (OCT2, BOB.1/OBF.1, PU.1) expression patterns in these cells corresponded to those of HRS cells in cHL. Based on analysis of 65 PTCLs, we have identified in the positive cases a highly significant increase of EBV+ small, reactive, resting B-cell compartment (75.9 / 100 HPF in PTCL vs. 1.5 / 100 HPF in control lymph nodes) likely to be due to the decreased immune surveillance. This progressive accumulation of EBV+ by-stander B-cell population in PTCLs might be the source of various B-cell proliferations, which in any form represent major diagnostic pitfalls and require a careful differential diagnostic procedure.

Original languageEnglish
Pages (from-to)377-383
Number of pages7
JournalPathology and Oncology Research
Volume16
Issue number3
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Peripheral T-Cell Lymphoma
Human Herpesvirus 4
Lymphoma
B-Lymphocytes
Reed-Sternberg Cells
B-Lymphoid Precursor Cells
Gene Rearrangement
Hodgkin Disease
Phenotype
Cell Proliferation
Immunoglobulin Heavy Chain Genes
Tissue Extracts
Lymphoma, Large B-Cell, Diffuse
In Situ Hybridization
Transcription Factors
Lymph Nodes
DNA
Population

Keywords

  • B-cell lymphoproliferation
  • Epstein-Barr virus
  • T-cell lymphoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Pheno- and genotypic features of epstein-barr virus associated B-cell lymphoproliferations in peripheral t-cell lymphomas. / Smuk, Gábor; Illés, A.; Keresztes, Katalin; Kereskai, L.; Márton, Balázs; Nagy, Zsófia; Lacza, Ágnes; Pajor, László.

In: Pathology and Oncology Research, Vol. 16, No. 3, 09.2010, p. 377-383.

Research output: Contribution to journalArticle

Smuk, Gábor ; Illés, A. ; Keresztes, Katalin ; Kereskai, L. ; Márton, Balázs ; Nagy, Zsófia ; Lacza, Ágnes ; Pajor, László. / Pheno- and genotypic features of epstein-barr virus associated B-cell lymphoproliferations in peripheral t-cell lymphomas. In: Pathology and Oncology Research. 2010 ; Vol. 16, No. 3. pp. 377-383.
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