Phasic, nonsynaptic GABA-A receptor-mediated inhibition entrains thalamocortical oscillations

Zita Rovó, Ferenc Mátyás, P. Barthó, Andrea Slézia, Sandro Lecci, Chiara Pellegrini, Simone Astori, Csaba Dávid, Balázs Hangya, Anita Lüthi, L. Acsády

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Abstract

GABA-A receptors (GABA-ARs) are typically expressed at synaptic or nonsynaptic sites mediating phasic and tonic inhibition, respectively. These two forms of inhibition conjointly control various network oscillations. To disentangle their roles in thalamocortical rhythms, we focally deleted synaptic, γ2 subunit-containing GABA-ARs in the thalamus using viral intervention in mice. After successful removal of γ2 subunit clusters, spontaneous and evoked GABAergic synaptic currents disappeared in thalamocortical cells when the presynaptic, reticular thalamic (nRT) neurons fired in tonic mode. However, when nRT cells fired in burst mode, slow phasic GABA-AR-mediated events persisted, indicating a dynamic, burst-specific recruitment of nonsynaptic GABA-ARs. In vivo, removal of synaptic GABA-ARs reduced the firing of individual thalamocortical cells but did not abolish slow oscillations or sleep spindles. We conclude that nonsynaptic GABA-ARs are recruited in a phasic manner specifically during burst firing of nRT cells and provide sufficient GABA-AR activation to control major thalamocortical oscillations.

Original languageEnglish
Pages (from-to)7137-7147
Number of pages11
JournalJournal of Neuroscience
Volume34
Issue number21
DOIs
Publication statusPublished - 2014

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GABA-A Receptors
Thalamus
gamma-Aminobutyric Acid
Inhibition (Psychology)
Sleep
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

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Phasic, nonsynaptic GABA-A receptor-mediated inhibition entrains thalamocortical oscillations. / Rovó, Zita; Mátyás, Ferenc; Barthó, P.; Slézia, Andrea; Lecci, Sandro; Pellegrini, Chiara; Astori, Simone; Dávid, Csaba; Hangya, Balázs; Lüthi, Anita; Acsády, L.

In: Journal of Neuroscience, Vol. 34, No. 21, 2014, p. 7137-7147.

Research output: Contribution to journalArticle

Rovó, Z, Mátyás, F, Barthó, P, Slézia, A, Lecci, S, Pellegrini, C, Astori, S, Dávid, C, Hangya, B, Lüthi, A & Acsády, L 2014, 'Phasic, nonsynaptic GABA-A receptor-mediated inhibition entrains thalamocortical oscillations', Journal of Neuroscience, vol. 34, no. 21, pp. 7137-7147. https://doi.org/10.1523/JNEUROSCI.4386-13.2014
Rovó, Zita ; Mátyás, Ferenc ; Barthó, P. ; Slézia, Andrea ; Lecci, Sandro ; Pellegrini, Chiara ; Astori, Simone ; Dávid, Csaba ; Hangya, Balázs ; Lüthi, Anita ; Acsády, L. / Phasic, nonsynaptic GABA-A receptor-mediated inhibition entrains thalamocortical oscillations. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 21. pp. 7137-7147.
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