Phase-fluorimetry study on dielectric relaxation of human serum albumin

Andrea Buzády, János Erostyák, Béla Somogyi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The dielectric relaxation (DR) of human serum albumin (HSA) was studied by the method of phase-fluorometry. The protein environment of the single tryptophan in HSA shows a relatively low-speed DR of sub-ns characteristic time. This relaxation can be measured as a decaying red-shift of the time-resolved fluorescence emission spectra. The details of calculations of time-emission matrices (TEM) and comparison to the fluorescence data of the reference solution of N-acetyl-l-tryptophanamide (NATA) are also presented. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)153-163
Number of pages11
JournalBiophysical Chemistry
Volume88
Issue number1-3
DOIs
Publication statusPublished - Dec 15 2000

Keywords

  • Dielectric relaxation
  • Human serum albumin
  • Phase-fluorometry
  • Time-emission matrix

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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