Pharmacological inhibition of Na/Ca exchange results in increased cellular Ca2+ load attributable to the predominance of forward mode block

Semir Ozdemir, Virginie Bito, Patricia Holemans, Laurent Vinet, Jean Jacques Mercadier, A. Varró, Karin R. Sipido

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Block of Na/Ca exchange (NCX) has potential therapeutic applications, in particular, if a mode-selective block could be achieved, but also carries serious risks for disturbing the normal Ca balance maintained by NCX. We have examined the effects of partial inhibition of NCX by SEA-0400 (1 or 0.3 μmol/L) in left ventricular myocytes from healthy pigs or mice and from mice with heart failure (MLP). During voltage clamp ramps with [Ca]i buffering, block of reverse mode block was slightly larger than of forward mode (by 25±5%, P

Original languageEnglish
Pages (from-to)1398-1405
Number of pages8
JournalCirculation Research
Volume102
Issue number11
DOIs
Publication statusPublished - Jun 6 2008

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Pharmacology
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Muscle Cells
Swine
Heart Failure
Therapeutics
SEA 0400

Keywords

  • Calcium overload
  • Cardiac myocytes
  • Heart failure
  • Na/Ca exchange
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Pharmacological inhibition of Na/Ca exchange results in increased cellular Ca2+ load attributable to the predominance of forward mode block. / Ozdemir, Semir; Bito, Virginie; Holemans, Patricia; Vinet, Laurent; Mercadier, Jean Jacques; Varró, A.; Sipido, Karin R.

In: Circulation Research, Vol. 102, No. 11, 06.06.2008, p. 1398-1405.

Research output: Contribution to journalArticle

Ozdemir, Semir ; Bito, Virginie ; Holemans, Patricia ; Vinet, Laurent ; Mercadier, Jean Jacques ; Varró, A. ; Sipido, Karin R. / Pharmacological inhibition of Na/Ca exchange results in increased cellular Ca2+ load attributable to the predominance of forward mode block. In: Circulation Research. 2008 ; Vol. 102, No. 11. pp. 1398-1405.
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