Pharmacological characterization of phospholipid growth-factor receptors

Gabor Tigyi, David J. Fischer, Daniel Baker, De An Wang, Junming Yue, Nora Nusser, Tamas Virag, Veronika Zsiros, K. Liliom, Duane Miller, Abby Parrill

Research output: Contribution to journalArticle

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Abstract

The phospholipid growth-factor (PLGF) terminology is proposed to describe a group of endogenous glycerol- and sphingolipid mediators that regulate cell proliferation through plasma membrane receptors. In addition to LPA and SPP, multiple PLGFs are present in blood plasma and serum, PLGF activity is regulated by its stimulus-coupled production and by endogenous inhibitors. In addition to LPA and SPP, alkenyl-glycerophosphate, cyclic-phosphatidic acid, and sphingosylphosphorylcholine were detected in biological fluids using mass spectrometry. Heterologous desensitization studies indicate the expression of multiple LPA-activated receptors in a variety of cell types, which are differentially activated by the different PLGFs. Northern blot and RT-PCR results reinforce the coexpression of PSP24α and different members of the EDG1-7 receptors in the same cell. Stable heterologous expression of the PSP24α, EDG2, and EDG4 receptors in HEK293 cells show distinct PLGF specificities and dose-response properties for each receptor subtype. Thus, both the controlled availability of the different agonists/inhibitors and the regulated expression of their receptors regulate the biological effects of PLGFs.

Original languageEnglish
Pages (from-to)34-53
Number of pages20
JournalAnnals of the New York Academy of Sciences
Volume905
Publication statusPublished - 2000

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Growth Factor Receptors
Phospholipids
Intercellular Signaling Peptides and Proteins
Pharmacology
Lysophosphatidic Acid Receptors
Glycerophosphates
Phosphatidic Acids
Sphingolipids
HEK293 Cells
Cell proliferation
Cell membranes
Terminology
Northern Blotting
Glycerol
Mass spectrometry
Mass Spectrometry
Blood
Cell Proliferation
Cell Membrane
Availability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tigyi, G., Fischer, D. J., Baker, D., Wang, D. A., Yue, J., Nusser, N., ... Parrill, A. (2000). Pharmacological characterization of phospholipid growth-factor receptors. Annals of the New York Academy of Sciences, 905, 34-53.

Pharmacological characterization of phospholipid growth-factor receptors. / Tigyi, Gabor; Fischer, David J.; Baker, Daniel; Wang, De An; Yue, Junming; Nusser, Nora; Virag, Tamas; Zsiros, Veronika; Liliom, K.; Miller, Duane; Parrill, Abby.

In: Annals of the New York Academy of Sciences, Vol. 905, 2000, p. 34-53.

Research output: Contribution to journalArticle

Tigyi, G, Fischer, DJ, Baker, D, Wang, DA, Yue, J, Nusser, N, Virag, T, Zsiros, V, Liliom, K, Miller, D & Parrill, A 2000, 'Pharmacological characterization of phospholipid growth-factor receptors', Annals of the New York Academy of Sciences, vol. 905, pp. 34-53.
Tigyi G, Fischer DJ, Baker D, Wang DA, Yue J, Nusser N et al. Pharmacological characterization of phospholipid growth-factor receptors. Annals of the New York Academy of Sciences. 2000;905:34-53.
Tigyi, Gabor ; Fischer, David J. ; Baker, Daniel ; Wang, De An ; Yue, Junming ; Nusser, Nora ; Virag, Tamas ; Zsiros, Veronika ; Liliom, K. ; Miller, Duane ; Parrill, Abby. / Pharmacological characterization of phospholipid growth-factor receptors. In: Annals of the New York Academy of Sciences. 2000 ; Vol. 905. pp. 34-53.
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