A new radiometric assay for measurement of phagocytosis of opsonized Saccharomyces cerevisiae by human mononuclear cells and granulocytes is presented. This assay was based upon the incorporation of 75Se-L-selenomethionine of the extracellular yeast cells after the ingestion step without a separation procedure for intracellular and extracellular microorganisms. Therefore the assay is objective, not time consuming and convenient for clinical application. After standardization of the method, in a preliminary study phagocytosis was evaluated in 44 individuals: 16 healthy controls, 13 patients with Hodgkin's disease and 15 patients with non-Hodgkin-lymphoma before treatment. In comparison to normal controls the phagocytosis in the mononuclear cell fraction of cancer patients was increased and in granulocytes decreased. The most extensive phagocytosis activity was shown in the mononuclear cell fraction of patients with Hodgkin's disease.
|Translated title of the contribution||Phagocytosis measurement in Hodgkin's disease and Non-Hodgkin lymphoma-patients with a new radiometric assay|
|Number of pages||8|
|Journal||Allergie und Immunologie|
|Publication status||Published - Dec 1 1982|
ASJC Scopus subject areas
- Immunology and Allergy