Phage T7-inactivation test. A possibility of quantitative mutagenicity screening.

G. Rontó, I. Tarján, S. Gáspár

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A new method is described for the quantitative characterization of the genotoxic effect of chemicals. The method is based on the determination of the inactivation of bacteriophage T7 and on the application of a simple mathematical model valid for the processes during, or at least in the initial stage of the interaction of chemicals and phages. A value characteristic for the chemical is defined and it is determined from the inactivation kinetics. Typical inactivation kinetic curves and some problems of the application of the model as well as the mutagenicity index values determined for about 30 substances are presented. The substances examined have mutagenicity index values covering a range of six orders of magnitude. The obtained values are compared with the results of different mutagenicity/carcinogenicity tests and discussed on the basis of data in the literature. The presented method is proposed to be applied for quantitative mutagenicity screening of chemicals.

Original languageEnglish
Pages (from-to)275-285
Number of pages11
JournalPhysiological Chemistry and Physics and Medical NMR
Volume18
Issue number4
Publication statusPublished - 1986

Fingerprint

Bacteriophage T7
Bacteriophages
Screening
Carcinogenicity Tests
Mutagenicity Tests
Kinetics
Theoretical Models
Mathematical models

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Physiology
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Phage T7-inactivation test. A possibility of quantitative mutagenicity screening. / Rontó, G.; Tarján, I.; Gáspár, S.

In: Physiological Chemistry and Physics and Medical NMR, Vol. 18, No. 4, 1986, p. 275-285.

Research output: Contribution to journalArticle

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