PH wave-front propagation in the urea-urease reaction

Magdalena M. Wrobel, Tamás Bánsági, Stephen K. Scott, Annette F. Taylor, Chris O. Bounds, Arturo Carranzo, John A. Pojman

Research output: Contribution to journalArticle

21 Citations (Scopus)


The urease-catalyzed hydrolysis of urea displays feedback that results in a switch from acid (pH ∼3) to base (pH ∼9) after a controllable period of time (from 10 to >5000 s). Here we show that the spatially distributed reaction can support pH wave fronts propagating with a speed of the order of 0.1-1 mm min-1. The experimental results were reproduced qualitatively in reaction-diffusion simulations including a Michaelis-Menten expression for the urease reaction with a bell-shaped rate-pH dependence. However, this model fails to predict that at lower enzyme concentrations, the unstirred reaction does not always support fronts when the well-stirred reaction still rapidly switches to high pH.

Original languageEnglish
Pages (from-to)610-615
Number of pages6
JournalBiophysical journal
Issue number3
Publication statusPublished - Aug 8 2012

ASJC Scopus subject areas

  • Biophysics

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    Wrobel, M. M., Bánsági, T., Scott, S. K., Taylor, A. F., Bounds, C. O., Carranzo, A., & Pojman, J. A. (2012). PH wave-front propagation in the urea-urease reaction. Biophysical journal, 103(3), 610-615.