pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers

A. Agostiano, F. Mavelli, F. Milano, L. Giotta, M. Trotta, L. Nagy, P. Maróti

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Isolated and purified reaction centers (RC) from Rhodobacter sphaeroides R-26.1 were solubilised in detergent with excess quinone and external electron donors and illuminated in the presence of pyranine. The pH change accompanying the reaction center photocycle was monitored by recording the variation of the pyranine fluorescence intensity. Using QB-depleted reaction centers or blocking the photocycle with terbutryne strongly reduced the pH change. The usefulness and limits of this technique in monitoring the pH changes during the RC photocycle are also discussed.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalBioelectrochemistry
Volume63
Issue number1-2
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Photosynthetic Reaction Center Complex Proteins
Detergents
Fluorescent Dyes
Protons
Dyes
dyes
Fluorescence
protons
Electrons
Monitoring
probes
Rhodobacter sphaeroides
detergents
quinones
recording
fluorescence
pyranine
electrons
terbutryne
benzoquinone

Keywords

  • Fluorescent dye
  • Proton uptake
  • Pyranine
  • Rhodobacter sphaeroides

ASJC Scopus subject areas

  • Biophysics
  • Electrochemistry
  • Physical and Theoretical Chemistry

Cite this

pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers. / Agostiano, A.; Mavelli, F.; Milano, F.; Giotta, L.; Trotta, M.; Nagy, L.; Maróti, P.

In: Bioelectrochemistry, Vol. 63, No. 1-2, 06.2004, p. 125-128.

Research output: Contribution to journalArticle

Agostiano, A. ; Mavelli, F. ; Milano, F. ; Giotta, L. ; Trotta, M. ; Nagy, L. ; Maróti, P. / pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers. In: Bioelectrochemistry. 2004 ; Vol. 63, No. 1-2. pp. 125-128.
@article{948cd5080eee4edf90bcbfe9ea67707a,
title = "pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers",
abstract = "Isolated and purified reaction centers (RC) from Rhodobacter sphaeroides R-26.1 were solubilised in detergent with excess quinone and external electron donors and illuminated in the presence of pyranine. The pH change accompanying the reaction center photocycle was monitored by recording the variation of the pyranine fluorescence intensity. Using QB-depleted reaction centers or blocking the photocycle with terbutryne strongly reduced the pH change. The usefulness and limits of this technique in monitoring the pH changes during the RC photocycle are also discussed.",
keywords = "Fluorescent dye, Proton uptake, Pyranine, Rhodobacter sphaeroides",
author = "A. Agostiano and F. Mavelli and F. Milano and L. Giotta and M. Trotta and L. Nagy and P. Mar{\'o}ti",
year = "2004",
month = "6",
doi = "10.1016/j.bioelechem.2003.10.015",
language = "English",
volume = "63",
pages = "125--128",
journal = "Bioelectrochemistry",
issn = "1567-5394",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers

AU - Agostiano, A.

AU - Mavelli, F.

AU - Milano, F.

AU - Giotta, L.

AU - Trotta, M.

AU - Nagy, L.

AU - Maróti, P.

PY - 2004/6

Y1 - 2004/6

N2 - Isolated and purified reaction centers (RC) from Rhodobacter sphaeroides R-26.1 were solubilised in detergent with excess quinone and external electron donors and illuminated in the presence of pyranine. The pH change accompanying the reaction center photocycle was monitored by recording the variation of the pyranine fluorescence intensity. Using QB-depleted reaction centers or blocking the photocycle with terbutryne strongly reduced the pH change. The usefulness and limits of this technique in monitoring the pH changes during the RC photocycle are also discussed.

AB - Isolated and purified reaction centers (RC) from Rhodobacter sphaeroides R-26.1 were solubilised in detergent with excess quinone and external electron donors and illuminated in the presence of pyranine. The pH change accompanying the reaction center photocycle was monitored by recording the variation of the pyranine fluorescence intensity. Using QB-depleted reaction centers or blocking the photocycle with terbutryne strongly reduced the pH change. The usefulness and limits of this technique in monitoring the pH changes during the RC photocycle are also discussed.

KW - Fluorescent dye

KW - Proton uptake

KW - Pyranine

KW - Rhodobacter sphaeroides

UR - http://www.scopus.com/inward/record.url?scp=2042422308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2042422308&partnerID=8YFLogxK

U2 - 10.1016/j.bioelechem.2003.10.015

DO - 10.1016/j.bioelechem.2003.10.015

M3 - Article

VL - 63

SP - 125

EP - 128

JO - Bioelectrochemistry

JF - Bioelectrochemistry

SN - 1567-5394

IS - 1-2

ER -