Personal overpressured-layer chromatography (OPLC) basic system 50, flexible tool in analytical and semipreparative work

E. Mincsovics, Márta Garami, László Kecskés, Barnabás Tapa, Zoltán Végh, G. Kátay, E. Tyihák

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

A new automated overpressured-layer chromatographic (OPLC) system called the Personal OPLC Basic System 50 is suitable for analytical and semi-preparative separations. The automatic microprocessor-controlled system ensures rapid and reproducible off-line isocratic and stepwise gradient separations. High external pressure (5 MPa) makes the sorbent layer more homogeneous, yielding more efficient off-line separation compared with those by early Chrompres chambers. A theoretical plate height of 10-30 μm can be achieved on an analytical high-performance thin-layer chromatographic (HPTLC) layer made of irregular silica gel with an average particle size of 5 μm if an optimal linear velocity (20-40 mm/min) and a nonviscous solvent system are used. On an analytical layer of 3 μm spherical silica gel, a theoretical plate height of 6-15 μm can be reached. Rapid analytical separations of resveratrol (1555 s) and xanthine by one-(498 s) and two-directional (274 s) off-line developments were accomplished. On-line separation and detection combined with off-line sample application and fully on-line processing (including on-line sample application, separation, and detection) were fulfilled with a TLC plate for xanthine separation. Semipreparative isolation of xanthines was achieved through a fully on-line OPLC operating mode and scaled-up chromatography.

Original languageEnglish
Pages (from-to)587-598
Number of pages12
JournalJournal of AOAC International
Volume82
Issue number3
Publication statusPublished - May 1999

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Chromatography
chromatography
Xanthine
Silica Gel
Xanthines
Microcomputers
xanthine
Particle Size
silica gel
xanthines
gel
silica
Pressure
microprocessors
resveratrol
adsorbents
Sorbents
Microprocessor chips
particle size
Particle size

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Personal overpressured-layer chromatography (OPLC) basic system 50, flexible tool in analytical and semipreparative work. / Mincsovics, E.; Garami, Márta; Kecskés, László; Tapa, Barnabás; Végh, Zoltán; Kátay, G.; Tyihák, E.

In: Journal of AOAC International, Vol. 82, No. 3, 05.1999, p. 587-598.

Research output: Contribution to journalArticle

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