Peripheral nerve lesion-induced uptake and transport of choleragenoid by capsaicin-sensitive C-fibre spinal ganglion neurons

G. Janczó, P. Sántha, Krisztina Gecse

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the present experiments the effect of systemic capsaicin treatment on the retrograde labelling of sensory ganglion cells was studied following the injection of choleratoxin B subunit-horseradish peroxidase conjugate (CTX-HRP) into intact and chronically transected peripheral nerves. In the control rats CTX-HRP injected into intact sciatic nerves labelled medium and large neurons with a mean cross-sectional area of 1041 ± 39 μm2. However, after injection of the conjugate into chronically transected sciatic nerves of the control rats, many small cells were also labelled, shifting the mean cross-sectional area of the labelled cells to 632 ± 118 μm2. Capsaicin pretreatment per se induced a moderate but significant decrease in the mean cross-sectional area of the labelled neurons (879 ± 79 μm2). More importantly, systemic pretreatment with capsaicin prevented the peripheral nerve lesion-induced labelling of small cells. Thus, the mean cross-sectional areas of labelled neurons relating to the intact and transected sciatic nerves, respectively, did not differ significantly. These findings provide direct evidence for a phenotypic switch of capsaicin-sensitive nociceptive neurons after peripheral nerve injury, and suggest that lesion-induced morphological changes in the spinal cord may be related to specific alterations in the chemistry of C-fibre afferent neurons rather than to a sprouting response of A-fibre afferents.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalActa Biologica Hungarica
Volume53
Issue number1-2
DOIs
Publication statusPublished - 2002

Fingerprint

Unmyelinated Nerve Fibers
capsaicin
peripheral nerves
Cholera Toxin
Capsaicin
Spinal Ganglia
Peripheral Nerves
lesion
lesions (animal)
Neurons
Sciatic Nerve
neurons
uptake mechanisms
nerve tissue
Rat control
Fibers
Horseradish Peroxidase
peroxidase
pretreatment
Labeling

Keywords

  • Capsaicin
  • Choleragenoid
  • Neuroplasticity
  • Peripheral nerve section
  • Sensory ganglion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Peripheral nerve lesion-induced uptake and transport of choleragenoid by capsaicin-sensitive C-fibre spinal ganglion neurons. / Janczó, G.; Sántha, P.; Gecse, Krisztina.

In: Acta Biologica Hungarica, Vol. 53, No. 1-2, 2002, p. 77-84.

Research output: Contribution to journalArticle

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