Peripheral inflammatory activation after hippocampus irradiation in the rat

Tünde Tokés, Gabriella Varga, Dénes Garab, Zoltán Nagy, Gábor Fekete, Eszter Tuboly, Imola Plangár, Imola Mán, Rita Emília Szabó, Zoltán Szabó, Gábor Volford, M. Ghyczy-, J. Kaszaki, M. Borós, K. Hideghéty

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: To detect the possible biochemical signs of inflammatory activation in the peripheral circulation in a rodent model of hippocampus irradiation, and to examine the effects of L-alpha-glycerylphosphorylcholine (GPC) in this experimental protocol. Materials and methods: Anesthetized Sprague-Dawley rats were subjected to 40 Gy cobalt irradiation of both hemispheres of the hippocampus, with or without GPC treatment (50 mg/kg intravenously (i.v.), 5 min before the irradiation, n = 6, each). A third group (n = 6) served as saline-treated control. Blood samples were obtained 3 h after the end of irradiation in order to examine the changes in plasma histamine, tumor necrosis factor-alpha (TNF-α), interleukin 1-beta, interleukin 6 (IL-6) and interleukin 10 (IL-10); liver tissue samples were taken to determine adenosine triphosphate (ATP) concentrations. Results: The hepatic ATP levels were significantly declined, while plasma concentrations of circulating TNF-α, IL-6, IL-10 and histamine were significantly increased after hippocampus irradiation. GPC treatment significantly reduced the irradiation-induced release of cytokines and histamine, and the liver ATP level was maintained at the control value. Conclusions: Targeted brain irradiation produced measurable pro-and anti-inflammatory cytokine changes in the systemic circulation. GPC supplementation provides significant protection against irradiation-induced peripheral pro-inflammatory activation and ATP depletion.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalInternational Journal of Radiation Biology
Volume90
Issue number1
DOIs
Publication statusPublished - Jan 2014

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Hippocampus
Adenosine Triphosphate
Interleukin-10
Histamine
Liver
Interleukin-6
Tumor Necrosis Factor-alpha
Glycerylphosphorylcholine
Cytokines
Histamine Release
Cobalt
Interleukin-1beta
Sprague Dawley Rats
Rodentia
Anti-Inflammatory Agents
Brain

Keywords

  • ATP
  • Brain irradiation
  • Cytokines
  • L-alpha-glycerylphosphorylcholine
  • Radioprotection
  • TNF-alpha

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Peripheral inflammatory activation after hippocampus irradiation in the rat. / Tokés, Tünde; Varga, Gabriella; Garab, Dénes; Nagy, Zoltán; Fekete, Gábor; Tuboly, Eszter; Plangár, Imola; Mán, Imola; Szabó, Rita Emília; Szabó, Zoltán; Volford, Gábor; Ghyczy-, M.; Kaszaki, J.; Borós, M.; Hideghéty, K.

In: International Journal of Radiation Biology, Vol. 90, No. 1, 01.2014, p. 1-6.

Research output: Contribution to journalArticle

Tokés, T, Varga, G, Garab, D, Nagy, Z, Fekete, G, Tuboly, E, Plangár, I, Mán, I, Szabó, RE, Szabó, Z, Volford, G, Ghyczy-, M, Kaszaki, J, Borós, M & Hideghéty, K 2014, 'Peripheral inflammatory activation after hippocampus irradiation in the rat', International Journal of Radiation Biology, vol. 90, no. 1, pp. 1-6. https://doi.org/10.3109/09553002.2013.836617
Tokés, Tünde ; Varga, Gabriella ; Garab, Dénes ; Nagy, Zoltán ; Fekete, Gábor ; Tuboly, Eszter ; Plangár, Imola ; Mán, Imola ; Szabó, Rita Emília ; Szabó, Zoltán ; Volford, Gábor ; Ghyczy-, M. ; Kaszaki, J. ; Borós, M. ; Hideghéty, K. / Peripheral inflammatory activation after hippocampus irradiation in the rat. In: International Journal of Radiation Biology. 2014 ; Vol. 90, No. 1. pp. 1-6.
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abstract = "Purpose: To detect the possible biochemical signs of inflammatory activation in the peripheral circulation in a rodent model of hippocampus irradiation, and to examine the effects of L-alpha-glycerylphosphorylcholine (GPC) in this experimental protocol. Materials and methods: Anesthetized Sprague-Dawley rats were subjected to 40 Gy cobalt irradiation of both hemispheres of the hippocampus, with or without GPC treatment (50 mg/kg intravenously (i.v.), 5 min before the irradiation, n = 6, each). A third group (n = 6) served as saline-treated control. Blood samples were obtained 3 h after the end of irradiation in order to examine the changes in plasma histamine, tumor necrosis factor-alpha (TNF-α), interleukin 1-beta, interleukin 6 (IL-6) and interleukin 10 (IL-10); liver tissue samples were taken to determine adenosine triphosphate (ATP) concentrations. Results: The hepatic ATP levels were significantly declined, while plasma concentrations of circulating TNF-α, IL-6, IL-10 and histamine were significantly increased after hippocampus irradiation. GPC treatment significantly reduced the irradiation-induced release of cytokines and histamine, and the liver ATP level was maintained at the control value. Conclusions: Targeted brain irradiation produced measurable pro-and anti-inflammatory cytokine changes in the systemic circulation. GPC supplementation provides significant protection against irradiation-induced peripheral pro-inflammatory activation and ATP depletion.",
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AU - Tuboly, Eszter

AU - Plangár, Imola

AU - Mán, Imola

AU - Szabó, Rita Emília

AU - Szabó, Zoltán

AU - Volford, Gábor

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AU - Kaszaki, J.

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N2 - Purpose: To detect the possible biochemical signs of inflammatory activation in the peripheral circulation in a rodent model of hippocampus irradiation, and to examine the effects of L-alpha-glycerylphosphorylcholine (GPC) in this experimental protocol. Materials and methods: Anesthetized Sprague-Dawley rats were subjected to 40 Gy cobalt irradiation of both hemispheres of the hippocampus, with or without GPC treatment (50 mg/kg intravenously (i.v.), 5 min before the irradiation, n = 6, each). A third group (n = 6) served as saline-treated control. Blood samples were obtained 3 h after the end of irradiation in order to examine the changes in plasma histamine, tumor necrosis factor-alpha (TNF-α), interleukin 1-beta, interleukin 6 (IL-6) and interleukin 10 (IL-10); liver tissue samples were taken to determine adenosine triphosphate (ATP) concentrations. Results: The hepatic ATP levels were significantly declined, while plasma concentrations of circulating TNF-α, IL-6, IL-10 and histamine were significantly increased after hippocampus irradiation. GPC treatment significantly reduced the irradiation-induced release of cytokines and histamine, and the liver ATP level was maintained at the control value. Conclusions: Targeted brain irradiation produced measurable pro-and anti-inflammatory cytokine changes in the systemic circulation. GPC supplementation provides significant protection against irradiation-induced peripheral pro-inflammatory activation and ATP depletion.

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