Peripheral cholinergic disturbances in Alzheimer's disease

Zoltán Rakonczay, Zoltán Horváth, A. Juhász, J. Kálmán

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The most pronounced neurochemical abnormality in Alzheimer's disease (AD) is cholinergic dysfunction in the central nervous system. Peripheral tissues may also be affected, however, including blood. The present study undertook to determine the activity of acetylcholinesterase (AChE) and its molecular forms in erythrocytes, lymphocytes and platelets of normal elderly subjects and probable AD cases. These samples contained dimeric globular (G2), tetrameric globular (G4) and asymmetric (A12) AChE forms, but no globular monomeric (G1) enzyme. In both lymphocytes and platelets, the major AChE molecular form was G2 (approximately 80%), with G4 and A12 forms accounting for nearly equal portions of the remainder. Total AChE activities and measured sedimentation coefficients were similar in the control and AD samples (from patients with mild and moderately severe cognitive deficiency). However, the groups differed significantly in the proportion of certain AChE molecular forms. Thus, as compared with controls, the amount of A12 AChE in the AD samples was increased 148 and 161% in lymphocytes and platelets, respectively. Genotyping for apolipoprotein E (ApoE) and the butyrylcholinesterase K (BCHE-K) variant, carried out using the polymerase chain reaction, showed that AD patients carried the ApoE4 allele at a significantly higher frequency than the controls. On the other hand there were no significant group differences in the occurrence of the BCHE-K variant and no synergism between ApoE alleles and the BCHE-K variant in our Hungarian AD population.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalChemico-Biological Interactions
Volume157-158
DOIs
Publication statusPublished - Dec 15 2005

Fingerprint

Acetylcholinesterase
Cholinergic Agents
Alzheimer Disease
Butyrylcholinesterase
compound A 12
Lymphocytes
Platelets
Blood Platelets
Apolipoproteins E
Alleles
Apolipoprotein E4
Polymerase chain reaction
Neurology
Sedimentation
Blood
Central Nervous System
Erythrocytes
Tissue
Polymerase Chain Reaction
Enzymes

Keywords

  • Acetylcholinesterase
  • Alzheimer's disease
  • Apolipoprotein E
  • Butyrylcholinesterase
  • Butyrylcholinesterase K variant
  • Molecular forms

ASJC Scopus subject areas

  • Toxicology

Cite this

Peripheral cholinergic disturbances in Alzheimer's disease. / Rakonczay, Zoltán; Horváth, Zoltán; Juhász, A.; Kálmán, J.

In: Chemico-Biological Interactions, Vol. 157-158, 15.12.2005, p. 233-238.

Research output: Contribution to journalArticle

Rakonczay, Zoltán ; Horváth, Zoltán ; Juhász, A. ; Kálmán, J. / Peripheral cholinergic disturbances in Alzheimer's disease. In: Chemico-Biological Interactions. 2005 ; Vol. 157-158. pp. 233-238.
@article{2562608490a64d53bf7f6c9301071bf7,
title = "Peripheral cholinergic disturbances in Alzheimer's disease",
abstract = "The most pronounced neurochemical abnormality in Alzheimer's disease (AD) is cholinergic dysfunction in the central nervous system. Peripheral tissues may also be affected, however, including blood. The present study undertook to determine the activity of acetylcholinesterase (AChE) and its molecular forms in erythrocytes, lymphocytes and platelets of normal elderly subjects and probable AD cases. These samples contained dimeric globular (G2), tetrameric globular (G4) and asymmetric (A12) AChE forms, but no globular monomeric (G1) enzyme. In both lymphocytes and platelets, the major AChE molecular form was G2 (approximately 80{\%}), with G4 and A12 forms accounting for nearly equal portions of the remainder. Total AChE activities and measured sedimentation coefficients were similar in the control and AD samples (from patients with mild and moderately severe cognitive deficiency). However, the groups differed significantly in the proportion of certain AChE molecular forms. Thus, as compared with controls, the amount of A12 AChE in the AD samples was increased 148 and 161{\%} in lymphocytes and platelets, respectively. Genotyping for apolipoprotein E (ApoE) and the butyrylcholinesterase K (BCHE-K) variant, carried out using the polymerase chain reaction, showed that AD patients carried the ApoE4 allele at a significantly higher frequency than the controls. On the other hand there were no significant group differences in the occurrence of the BCHE-K variant and no synergism between ApoE alleles and the BCHE-K variant in our Hungarian AD population.",
keywords = "Acetylcholinesterase, Alzheimer's disease, Apolipoprotein E, Butyrylcholinesterase, Butyrylcholinesterase K variant, Molecular forms",
author = "Zolt{\'a}n Rakonczay and Zolt{\'a}n Horv{\'a}th and A. Juh{\'a}sz and J. K{\'a}lm{\'a}n",
year = "2005",
month = "12",
day = "15",
doi = "10.1016/j.cbi.2005.10.034",
language = "English",
volume = "157-158",
pages = "233--238",
journal = "Chemico-Biological Interactions",
issn = "0009-2797",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Peripheral cholinergic disturbances in Alzheimer's disease

AU - Rakonczay, Zoltán

AU - Horváth, Zoltán

AU - Juhász, A.

AU - Kálmán, J.

PY - 2005/12/15

Y1 - 2005/12/15

N2 - The most pronounced neurochemical abnormality in Alzheimer's disease (AD) is cholinergic dysfunction in the central nervous system. Peripheral tissues may also be affected, however, including blood. The present study undertook to determine the activity of acetylcholinesterase (AChE) and its molecular forms in erythrocytes, lymphocytes and platelets of normal elderly subjects and probable AD cases. These samples contained dimeric globular (G2), tetrameric globular (G4) and asymmetric (A12) AChE forms, but no globular monomeric (G1) enzyme. In both lymphocytes and platelets, the major AChE molecular form was G2 (approximately 80%), with G4 and A12 forms accounting for nearly equal portions of the remainder. Total AChE activities and measured sedimentation coefficients were similar in the control and AD samples (from patients with mild and moderately severe cognitive deficiency). However, the groups differed significantly in the proportion of certain AChE molecular forms. Thus, as compared with controls, the amount of A12 AChE in the AD samples was increased 148 and 161% in lymphocytes and platelets, respectively. Genotyping for apolipoprotein E (ApoE) and the butyrylcholinesterase K (BCHE-K) variant, carried out using the polymerase chain reaction, showed that AD patients carried the ApoE4 allele at a significantly higher frequency than the controls. On the other hand there were no significant group differences in the occurrence of the BCHE-K variant and no synergism between ApoE alleles and the BCHE-K variant in our Hungarian AD population.

AB - The most pronounced neurochemical abnormality in Alzheimer's disease (AD) is cholinergic dysfunction in the central nervous system. Peripheral tissues may also be affected, however, including blood. The present study undertook to determine the activity of acetylcholinesterase (AChE) and its molecular forms in erythrocytes, lymphocytes and platelets of normal elderly subjects and probable AD cases. These samples contained dimeric globular (G2), tetrameric globular (G4) and asymmetric (A12) AChE forms, but no globular monomeric (G1) enzyme. In both lymphocytes and platelets, the major AChE molecular form was G2 (approximately 80%), with G4 and A12 forms accounting for nearly equal portions of the remainder. Total AChE activities and measured sedimentation coefficients were similar in the control and AD samples (from patients with mild and moderately severe cognitive deficiency). However, the groups differed significantly in the proportion of certain AChE molecular forms. Thus, as compared with controls, the amount of A12 AChE in the AD samples was increased 148 and 161% in lymphocytes and platelets, respectively. Genotyping for apolipoprotein E (ApoE) and the butyrylcholinesterase K (BCHE-K) variant, carried out using the polymerase chain reaction, showed that AD patients carried the ApoE4 allele at a significantly higher frequency than the controls. On the other hand there were no significant group differences in the occurrence of the BCHE-K variant and no synergism between ApoE alleles and the BCHE-K variant in our Hungarian AD population.

KW - Acetylcholinesterase

KW - Alzheimer's disease

KW - Apolipoprotein E

KW - Butyrylcholinesterase

KW - Butyrylcholinesterase K variant

KW - Molecular forms

UR - http://www.scopus.com/inward/record.url?scp=28744447843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28744447843&partnerID=8YFLogxK

U2 - 10.1016/j.cbi.2005.10.034

DO - 10.1016/j.cbi.2005.10.034

M3 - Article

VL - 157-158

SP - 233

EP - 238

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

SN - 0009-2797

ER -