Peripheral blood lymphocytes display reduced k+ channel activity in aged humans

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Abstract

Steady-state parameters of whole-cell K+ current have been determined in human peripheral blood lymphocytes of young (20-50 y.) and elderly (>90 y.) volunteers by patch-clamp. The magnitude and voltage dependence of the K+ conductance were similar in both lymphocyte populations. The midpoint of steady-state inactivation was -53.3 ± 2.3 mV for lymphocyte population of young individuals and -65.0 ± 3.0 mV for that of elderly, showing a significant shift to hyperpolarized potentials. The peak of the steady-state open probability of the K+ channels was decreased and shifted to depolarized potentials by approx. 12.5 mV for lymphocytes of elderly donors. It is suggested that the observed differences in the K+ current parameters may be at least partly responsible for the impaired responsiveness of elderly lymphocytes to proliferative stimuli.

Original languageEnglish
Pages (from-to)519-524
Number of pages6
JournalBiochemical and biophysical research communications
Volume199
Issue number2
DOIs
Publication statusPublished - Mar 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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