Peripheral blood fibrocytes contribute to the formation of the avian spleen

N. Nagy, Éva Bíro, A. Takács, Miklós Pólos, A. Magyar, Imre Oláh

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Chick-quail chimeric studies were made to determine the origin of the cells of splenic ellipsoid. The ellipsoid is formed by supporting and phagocytic cells, which are embedded in a well-organized extracellular matrix. Splenic and bursal anlage of 6- to 6.5-day-old quail embryos were transplanted into the coelomic cavity of 3-day-old chick embryos and further incubated for 17 days. CD45+ chicken hemopoietic cells colonized both organs. They formed the cells of the ellipsoid and the periellipsoidal white pulp of the transplanted quail spleen. Chicken-specific collagen III was produced only in the donor quail spleen, but not in the bursa of Fabricius. The CD45+/collagen I+/collagen III+ cells are probably identical with the mammalian peripheral blood fibrocytes and contribute to the formation of supporting cells, whereas the CD45+/74.2+ ellipsoid-associated macrophages are of monocytic origin. We provide, for the first time, experimental evidence that peripheral blood fibrocytes exist in the avian species; they are present in the circulation of the chicken embryo and contribute to the organogenesis of the spleen.

Original languageEnglish
Pages (from-to)55-66
Number of pages12
JournalDevelopmental Dynamics
Volume232
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Quail
Spleen
Chickens
Collagen
Embryonic Structures
Bursa of Fabricius
Organogenesis
Chick Embryo
Phagocytes
Extracellular Matrix
Macrophages

Keywords

  • Ellipsoid
  • Peripheral blood fibrocyte
  • Quail-chick chimera
  • Spleen
  • Splenic organogenesis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Peripheral blood fibrocytes contribute to the formation of the avian spleen. / Nagy, N.; Bíro, Éva; Takács, A.; Pólos, Miklós; Magyar, A.; Oláh, Imre.

In: Developmental Dynamics, Vol. 232, No. 1, 01.2005, p. 55-66.

Research output: Contribution to journalArticle

Nagy, N. ; Bíro, Éva ; Takács, A. ; Pólos, Miklós ; Magyar, A. ; Oláh, Imre. / Peripheral blood fibrocytes contribute to the formation of the avian spleen. In: Developmental Dynamics. 2005 ; Vol. 232, No. 1. pp. 55-66.
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