Peripheral and central actions of AF64A (ethylcholine mustard aziridinium ion) on acetylcholine release, in vitro: Comparison with hemicholinium

P. E. Potter, L. Hársing, I. Kakucska, G. Gaal, E. Vízi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The acute effects of ethylcholine mustard aziridinium ion (AF64A) and hemicholinium-3 (HC-3) on the release of endogenous acetylcholine (ACh) from isolated tissues were examined. Whereas addition of HC-3 (10-6-10-5 M) significantly reduced the output of ACh from isolated guinea-pig ileum longitudinal muscle strip elicited by 10 Hz stimulation, AF64A had no effect and even enhanced the release of radiolabel elicited by 1 Hz stimulation when this tissue was pre-loaded with [3H]choline. Similarly, HC-3 (10-5 M) reduced ouabain-induced endogenous ACh release from isolated rat hippocampus. Addition of AF64A (10-5-5 × 10-5 M) caused a slight increase in ACh release. In isolated rat cortex, however, AF64A did not affect ACh release. Moreover, AF64A caused a decrease in ouabain-stimulated ACh release from striatum. The present study indicates that: (a) the in vitro actions of AF64A differ from those of HC-3 and (b) the acute effects of AF64A on endogenous ACh release vary, depending on the tissues studied and the stimulation parameters used.

Original languageEnglish
Pages (from-to)1047-1053
Number of pages7
JournalNeurochemistry International
Volume7
Issue number6
DOIs
Publication statusPublished - 1985

Fingerprint

Hemicholinium 3
Acetylcholine
Ions
Ouabain
ethylcholine aziridinium
In Vitro Techniques
Choline
Ileum
Hippocampus
Guinea Pigs
Muscles

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Peripheral and central actions of AF64A (ethylcholine mustard aziridinium ion) on acetylcholine release, in vitro : Comparison with hemicholinium. / Potter, P. E.; Hársing, L.; Kakucska, I.; Gaal, G.; Vízi, E.

In: Neurochemistry International, Vol. 7, No. 6, 1985, p. 1047-1053.

Research output: Contribution to journalArticle

@article{a856605a74af43528eac81316a98c194,
title = "Peripheral and central actions of AF64A (ethylcholine mustard aziridinium ion) on acetylcholine release, in vitro: Comparison with hemicholinium",
abstract = "The acute effects of ethylcholine mustard aziridinium ion (AF64A) and hemicholinium-3 (HC-3) on the release of endogenous acetylcholine (ACh) from isolated tissues were examined. Whereas addition of HC-3 (10-6-10-5 M) significantly reduced the output of ACh from isolated guinea-pig ileum longitudinal muscle strip elicited by 10 Hz stimulation, AF64A had no effect and even enhanced the release of radiolabel elicited by 1 Hz stimulation when this tissue was pre-loaded with [3H]choline. Similarly, HC-3 (10-5 M) reduced ouabain-induced endogenous ACh release from isolated rat hippocampus. Addition of AF64A (10-5-5 × 10-5 M) caused a slight increase in ACh release. In isolated rat cortex, however, AF64A did not affect ACh release. Moreover, AF64A caused a decrease in ouabain-stimulated ACh release from striatum. The present study indicates that: (a) the in vitro actions of AF64A differ from those of HC-3 and (b) the acute effects of AF64A on endogenous ACh release vary, depending on the tissues studied and the stimulation parameters used.",
author = "Potter, {P. E.} and L. H{\'a}rsing and I. Kakucska and G. Gaal and E. V{\'i}zi",
year = "1985",
doi = "10.1016/0197-0186(85)90154-8",
language = "English",
volume = "7",
pages = "1047--1053",
journal = "Neurochemistry International",
issn = "0197-0186",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Peripheral and central actions of AF64A (ethylcholine mustard aziridinium ion) on acetylcholine release, in vitro

T2 - Comparison with hemicholinium

AU - Potter, P. E.

AU - Hársing, L.

AU - Kakucska, I.

AU - Gaal, G.

AU - Vízi, E.

PY - 1985

Y1 - 1985

N2 - The acute effects of ethylcholine mustard aziridinium ion (AF64A) and hemicholinium-3 (HC-3) on the release of endogenous acetylcholine (ACh) from isolated tissues were examined. Whereas addition of HC-3 (10-6-10-5 M) significantly reduced the output of ACh from isolated guinea-pig ileum longitudinal muscle strip elicited by 10 Hz stimulation, AF64A had no effect and even enhanced the release of radiolabel elicited by 1 Hz stimulation when this tissue was pre-loaded with [3H]choline. Similarly, HC-3 (10-5 M) reduced ouabain-induced endogenous ACh release from isolated rat hippocampus. Addition of AF64A (10-5-5 × 10-5 M) caused a slight increase in ACh release. In isolated rat cortex, however, AF64A did not affect ACh release. Moreover, AF64A caused a decrease in ouabain-stimulated ACh release from striatum. The present study indicates that: (a) the in vitro actions of AF64A differ from those of HC-3 and (b) the acute effects of AF64A on endogenous ACh release vary, depending on the tissues studied and the stimulation parameters used.

AB - The acute effects of ethylcholine mustard aziridinium ion (AF64A) and hemicholinium-3 (HC-3) on the release of endogenous acetylcholine (ACh) from isolated tissues were examined. Whereas addition of HC-3 (10-6-10-5 M) significantly reduced the output of ACh from isolated guinea-pig ileum longitudinal muscle strip elicited by 10 Hz stimulation, AF64A had no effect and even enhanced the release of radiolabel elicited by 1 Hz stimulation when this tissue was pre-loaded with [3H]choline. Similarly, HC-3 (10-5 M) reduced ouabain-induced endogenous ACh release from isolated rat hippocampus. Addition of AF64A (10-5-5 × 10-5 M) caused a slight increase in ACh release. In isolated rat cortex, however, AF64A did not affect ACh release. Moreover, AF64A caused a decrease in ouabain-stimulated ACh release from striatum. The present study indicates that: (a) the in vitro actions of AF64A differ from those of HC-3 and (b) the acute effects of AF64A on endogenous ACh release vary, depending on the tissues studied and the stimulation parameters used.

UR - http://www.scopus.com/inward/record.url?scp=0022380002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022380002&partnerID=8YFLogxK

U2 - 10.1016/0197-0186(85)90154-8

DO - 10.1016/0197-0186(85)90154-8

M3 - Article

AN - SCOPUS:0022380002

VL - 7

SP - 1047

EP - 1053

JO - Neurochemistry International

JF - Neurochemistry International

SN - 0197-0186

IS - 6

ER -