Periosteal microcirculatory reactions in a zoledronate-induced osteonecrosis model of the jaw in rats

Ágnes Janovszky, Andrea Szabó, Renáta Varga, Dénes Garab, M. Borós, Csilla Mester, Nikolett Beretka, Tamás Zombori, Hans Peter Wiesmann, Ricardo Bernhardt, I. Ocsovszki, Péter Balázs, József Piffkó

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: Nitrogen-containing bisphosphonates induce osteonecrosis mostly in the jaw and less frequently in other bones. Because of the crucial role of periosteal perfusion in bone repair, we investigated zoledronate-induced microcirculatory reactions in the mandibular periosteum in comparison with those in the tibia in a clinically relevant model of bisphosphonate-induced medication-related osteonecrosis of the jaw (MRONJ). Materials and methods: Sprague–Dawley rats were treated with zoledronate (ZOL; 80 i.v. μg/kg/week over 8 weeks) or saline vehicle. The first two right mandibular molar teeth were extracted after 3 weeks. Various systemic and local (periosteal) microcirculatory inflammatory parameters were examined by intravital videomicroscopy after 9 weeks. Results: Gingival healing disorders (∼100 %) and MRONJ developed in 70 % of ZOL-treated cases but not after saline (shown by micro-CT). ZOL induced significantly higher degrees of periosteal leukocyte rolling and adhesion in the mandibular postcapillary venules (at both extraction and intact sites) than at the tibia. Leukocyte NADPH-oxidase activity was reduced; leukocyte CD11b and plasma TNF-alpha levels were unchanged. Conclusion: Chronic ZOL treatment causes a distinct microcirculatory inflammatory reaction in the mandibular periosteum but not in the tibia. The local reaction in the absence of augmented systemic leukocyte inflammatory activity suggests that topically different, endothelium-specific changes may play a critical role in the pathogenesis of MRONJ. Clinical relevance: This model permits for the first time to explore the microvascular processes in the mandibular periosteum after chronic ZOL treatment. This approach may contribute to a better understanding of the pathomechanism and the development of strategies to counteract bisphosphonate-induced side effects.

Original languageEnglish
Pages (from-to)1279-1288
Number of pages10
JournalClinical Oral Investigations
Volume19
Issue number6
DOIs
Publication statusPublished - Oct 30 2014

Fingerprint

zoledronic acid
Osteonecrosis
Jaw
Periosteum
Tibia
Leukocytes
Diphosphonates
Bisphosphonate-Associated Osteonecrosis of the Jaw
Leukocyte Rolling
Bone and Bones
Video Microscopy
Venules
NADPH Oxidase
Endothelium
Tooth
Nitrogen
Tumor Necrosis Factor-alpha
Perfusion
Therapeutics

Keywords

  • Bisphosphonate
  • Inflammation
  • Intravital fluorescence videomicroscopy
  • Leukocytes
  • Mandibular periosteum
  • Osteonecrosis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Periosteal microcirculatory reactions in a zoledronate-induced osteonecrosis model of the jaw in rats. / Janovszky, Ágnes; Szabó, Andrea; Varga, Renáta; Garab, Dénes; Borós, M.; Mester, Csilla; Beretka, Nikolett; Zombori, Tamás; Wiesmann, Hans Peter; Bernhardt, Ricardo; Ocsovszki, I.; Balázs, Péter; Piffkó, József.

In: Clinical Oral Investigations, Vol. 19, No. 6, 30.10.2014, p. 1279-1288.

Research output: Contribution to journalArticle

Janovszky, Á, Szabó, A, Varga, R, Garab, D, Borós, M, Mester, C, Beretka, N, Zombori, T, Wiesmann, HP, Bernhardt, R, Ocsovszki, I, Balázs, P & Piffkó, J 2014, 'Periosteal microcirculatory reactions in a zoledronate-induced osteonecrosis model of the jaw in rats', Clinical Oral Investigations, vol. 19, no. 6, pp. 1279-1288. https://doi.org/10.1007/s00784-014-1347-6
Janovszky, Ágnes ; Szabó, Andrea ; Varga, Renáta ; Garab, Dénes ; Borós, M. ; Mester, Csilla ; Beretka, Nikolett ; Zombori, Tamás ; Wiesmann, Hans Peter ; Bernhardt, Ricardo ; Ocsovszki, I. ; Balázs, Péter ; Piffkó, József. / Periosteal microcirculatory reactions in a zoledronate-induced osteonecrosis model of the jaw in rats. In: Clinical Oral Investigations. 2014 ; Vol. 19, No. 6. pp. 1279-1288.
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AU - Szabó, Andrea

AU - Varga, Renáta

AU - Garab, Dénes

AU - Borós, M.

AU - Mester, Csilla

AU - Beretka, Nikolett

AU - Zombori, Tamás

AU - Wiesmann, Hans Peter

AU - Bernhardt, Ricardo

AU - Ocsovszki, I.

AU - Balázs, Péter

AU - Piffkó, József

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N2 - Objectives: Nitrogen-containing bisphosphonates induce osteonecrosis mostly in the jaw and less frequently in other bones. Because of the crucial role of periosteal perfusion in bone repair, we investigated zoledronate-induced microcirculatory reactions in the mandibular periosteum in comparison with those in the tibia in a clinically relevant model of bisphosphonate-induced medication-related osteonecrosis of the jaw (MRONJ). Materials and methods: Sprague–Dawley rats were treated with zoledronate (ZOL; 80 i.v. μg/kg/week over 8 weeks) or saline vehicle. The first two right mandibular molar teeth were extracted after 3 weeks. Various systemic and local (periosteal) microcirculatory inflammatory parameters were examined by intravital videomicroscopy after 9 weeks. Results: Gingival healing disorders (∼100 %) and MRONJ developed in 70 % of ZOL-treated cases but not after saline (shown by micro-CT). ZOL induced significantly higher degrees of periosteal leukocyte rolling and adhesion in the mandibular postcapillary venules (at both extraction and intact sites) than at the tibia. Leukocyte NADPH-oxidase activity was reduced; leukocyte CD11b and plasma TNF-alpha levels were unchanged. Conclusion: Chronic ZOL treatment causes a distinct microcirculatory inflammatory reaction in the mandibular periosteum but not in the tibia. The local reaction in the absence of augmented systemic leukocyte inflammatory activity suggests that topically different, endothelium-specific changes may play a critical role in the pathogenesis of MRONJ. Clinical relevance: This model permits for the first time to explore the microvascular processes in the mandibular periosteum after chronic ZOL treatment. This approach may contribute to a better understanding of the pathomechanism and the development of strategies to counteract bisphosphonate-induced side effects.

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KW - Intravital fluorescence videomicroscopy

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KW - Mandibular periosteum

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