Peptides with antimicrobial and anti-inflammatory activities that have therapeutic potential for treatment of acne vulgaris

Suzana Popovic, E. Urbán, Miodrag Lukic, J. Michael Conlon

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The pathogenesis of acne vulgaris is multifactorial involving infection of the pilosebaceous unit with Propionibacterium acnes and a cytokine-mediated inflammatory response. Five frog skin-derived antimicrobial peptides ([D4k]ascaphin-8, [G4K]XT-7, [T5k]temporin-DRa, brevinin-2GU, and B2RP-ERa), chosen for their low hemolytic activity against human erythrocytes, were assessed for their effects on the growth of clinical isolates of P. acnes and on the release of pro-inflammatory and anti-inflammatory cytokines from peripheral blood mononuclear (PBM) cells. All peptides inhibited the growth of P. acnes with the highest potency exhibited by [D4k]ascaphin-8 (minimum inhibitory concentration, MIC = 3-12.5 μM). Release of TNF-α from concanavalin A (ConA)-stimulated PBM cells was significantly reduced by [D4k]ascaphin-8, [G4K]XT-7, brevinin-2GU, and B2RP-ERa (1 and 20 μg/ml) and by [T5k]temporin-DRa (20 μg/ml). Release of IFN-γ from unstimulated PBM cells was significantly reduced by [D4k]ascaphin-8 and brevinin-2GU (1 and 20 μg/ml). No peptide showed significant effects on Il-17 release. Release of the anti-inflammatory cytokines TGF-β, IL-4, and IL-10 from both unstimulated and ConA-treated PBM cells was significantly increased by [T5k]temporin-DRa and B2RP-ERa (1 and 20 μg/ml). The potent activities of [D4k]ascaphin-8 and [T5k]temporin-DRa in inhibiting the growth of P. acnes and the release of pro-inflammatory cytokines, and in stimulating the release of anti-inflammatory cytokines suggest a possible therapeutic role in the treatment of acne vulgaris.

Original languageEnglish
Pages (from-to)275-282
Number of pages8
JournalPeptides
Volume34
Issue number2
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Acne Vulgaris
Propionibacterium acnes
Anti-Inflammatory Agents
Cytokines
Blood Cells
Blood
Peptides
Concanavalin A
Growth
Therapeutics
Microbial Sensitivity Tests
Human Activities
Interleukin-4
Anura
Interleukin-10
Skin
Erythrocytes
temporin
Infection

Keywords

  • Acne vulgaris
  • Cytokine
  • Frog skin antimicrobial peptide
  • Propionibacterium acnes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Peptides with antimicrobial and anti-inflammatory activities that have therapeutic potential for treatment of acne vulgaris. / Popovic, Suzana; Urbán, E.; Lukic, Miodrag; Conlon, J. Michael.

In: Peptides, Vol. 34, No. 2, 04.2012, p. 275-282.

Research output: Contribution to journalArticle

Popovic, Suzana ; Urbán, E. ; Lukic, Miodrag ; Conlon, J. Michael. / Peptides with antimicrobial and anti-inflammatory activities that have therapeutic potential for treatment of acne vulgaris. In: Peptides. 2012 ; Vol. 34, No. 2. pp. 275-282.
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