Peptidergic neurons in the snail Helix pomatia: Distribution of neurons in the central and peripheral nervous systems that react with an Antibody raised to the insect neuropeptide, leucokinin I

K. Elekes, L. Hernádi, J. E. Muren, D. R. Nässel

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In this study, antiserum raised against an insect myotropic peptide, leucokinin I (DPAFNSWGamide), was: used for mapping leucokinin‐like immunoreactive (LK‐LI) neurons in the gastropod mollusc, Helix pomatia. Immunocytochemistry performed on both whole‐mounts and cryostat sections demonstrated LK‐LI neurons in all ganglia of the central nervous system (CNS), except the visceral ganglion. Altogether about 700 immunolabelled neurons have been found, with nearly one‐half (46%) in the cerebral ganglia. A large proportion of the LK‐LI neurons have small cell bodies and are likely to be interneurons. The most prominent LK‐LI cell group is represented by the entire neuron population of the mesocerebri, which is the major source of a thick fiber bundle system, encircling and innervating the whole CNS. One single LK‐LI giant neuron was found, which is located in the left pedal ganglion and is termed GLPdLKC (giant left pedal leucokinin immunoreactive cell). This cell has not been identified previously. The ganglion neuropils are heavily innervated by varicose LK‐LI fiber arborizations. Some integrative centers, such as the medullary neuropil of the procerebri, reveal an extreme density of LK‐LI innervation. All major peripheral nerves contain a large number of LK‐LI axons, and LK‐LI innervation is found in the musculature of different peripheral organs (buccal mass, lip, tentacles, oviduct, intestine). Among the peripheral organs investigated, the intestine contains a rich varicose LK‐LI network, composed of both intrinsic and extrinsic elements. Radioimmunoassay (RIA) demonstrates a very high content of LK‐LI material in Helix ganglion extracts (about 50 pmol/CNS). This is the first report on the occurrence of a substance resembling the myotropic neuropeptide leucokinin I in a phylum outside arthropods. Based on our immunocytochemical observations, a role for leucokinin‐like peptides in both central and peripheral regulatory processes in Helix is suggested. According to double‐labelling experiments, only a small number of the LK‐LI neurons are labelled with an antibody to the vertebrate tachykinin substance P.

Original languageEnglish
Pages (from-to)257-272
Number of pages16
JournalJournal of Comparative Neurology
Volume341
Issue number2
DOIs
Publication statusPublished - Mar 8 1994

Keywords

  • central
  • gastropod mollusc
  • immunocytochemistry
  • myotropic neuropeptide
  • nervous system
  • peripheral
  • tachykinins

ASJC Scopus subject areas

  • Neuroscience(all)

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