Pentosan polysulfate regulates scavenger receptor-mediated, but not fluid-phase, endocytosis in immortalized cerebral endothelial cells

M. Deli, C. Ábrahám, Hideaki Takahata, Shigeru Katamine, Masami Niwa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

1. Effects of pentosan polysulfate (PPS) and the structurally related sulfated polyanions dextran sulfate, fucoidan, and heparin on the scavenger receptor-mediated and fluid-phase endocytosis in GP8 immortalized rat brain endothelial cells were investigated. 2. Using 1,1'-dioctadecyl-3,3,3,3'-tetramethylindocarboxyamine perchlorate-labeled acetylated low-density lipoprotein (DiI-AcLDL), we found a binding site with high affinity and low binding capacity, and another one with low affinity and high binding capacity. Increasing ligand concentrations could not saturate DiI-AcLDL uptake. DiI-AcLDL uptake, but not binding, was sensitive to pretreatment with filipin, an inhibitor of caveola formation. 3. PPS (20-200 μg/ml) significantly reduced the binding of DiI-AcLDL after coincubation for 3 hr, though this effect was less expressed after 18 hr. Among other polyanions, only fucoidan decreased the DiI-AcLDL binding after 3 hr, whereas dextran sulfate significantly increased it after 18 hr. PPS treatment induced an increase in DiI-AcLDL uptake, whereas other polysulfated compounds caused a significant reduction. 4. Fluid-phase endocytosis determined by the accumulation of Lucifer yellow was concentration and time dependent in GP8 cells. Coincubation with PPS or other sulfated polyanions could not significantly alter the rate of Lucifer yellow uptake. 5. In conclusion, PPS decreased the binding and increased the uptake of DiI-AcLDL in cerebral endothelial cells, an effect not mimicked by the other polyanions investigated.

Original languageEnglish
Pages (from-to)731-745
Number of pages15
JournalCellular and Molecular Neurobiology
Volume20
Issue number6
DOIs
Publication statusPublished - 2000

Fingerprint

Pentosan Sulfuric Polyester
Scavenger Receptors
Endothelial cells
Endocytosis
Endothelial Cells
Fluids
Dextran Sulfate
Filipin
Caveolae
acetyl-LDL
perchlorate
Heparin
Rats
Brain
Binding Sites
Ligands
polyanions

Keywords

  • Blood-brain barrier
  • Dextran sulfate
  • Fluid-phase endocytosis
  • Fucoidan
  • Heparin
  • Pentosan polysulfate
  • Receptor-mediated endocytosis
  • Scavenger receptors

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Pentosan polysulfate regulates scavenger receptor-mediated, but not fluid-phase, endocytosis in immortalized cerebral endothelial cells. / Deli, M.; Ábrahám, C.; Takahata, Hideaki; Katamine, Shigeru; Niwa, Masami.

In: Cellular and Molecular Neurobiology, Vol. 20, No. 6, 2000, p. 731-745.

Research output: Contribution to journalArticle

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